EMAGE mouse gene expression spatial database

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EMAGE:7878

Tmem145 transmembrane protein 145 ( MGI:3607779)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012829. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012829_01	euxassay_012829_02	euxassay_012829_03	euxassay_012829_04

euxassay_012829_05	euxassay_012829_06	euxassay_012829_07	euxassay_012829_08	euxassay_012829_09

euxassay_012829_10	euxassay_012829_11	euxassay_012829_12	euxassay_012829_13	euxassay_012829_14

euxassay_012829_15	euxassay_012829_16	euxassay_012829_17	euxassay_012829_18	euxassay_012829_19

euxassay_012829_20	euxassay_012829_21	euxassay_012829_22	euxassay_012829_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7878_wholemount_strong.wlz

7878_wholemount_moderate.wlz

7878_wholemount_weak.wlz

7878_wholemount_possible.wlz

7878_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7878_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 08 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 05 21 22 23 weak expression: see section 03 04 07 08 09 10 11 12 13 14 15 16 17 18 19

olfactory cortex marginal layer

weak

regional

weak expression: see section 11 12 16 17 18

medulla oblongata alar plate mantle layer

moderate

homogeneous

moderate expression: see section 07 09 10 11 14 15 16 17 weak expression: see section 08 12 13

medulla oblongata basal plate mantle layer

moderate

homogeneous

moderate expression: see section 07 09 10 11 14 15 16 17 weak expression: see section 06 08 12 13

rest of cerebellum mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 04 05 06 07 08 19 20 21

pons mantle layer

moderate

homogeneous

moderate expression: see section 07 09 10 11 14 15 16 17 18 19 weak expression: see section 06 08 12 13

midbrain mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 06 07 08 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 05 21 weak expression: see section 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 21 weak expression: see section 03 04 06 07 08 09 17 18 19 20 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 17

spinal cord mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 14 weak expression: see section 08 12 13

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38179
Entity Detected:	Tmem145, transmembrane protein 145 ( MGI:3607779)
Sequence:	sense strand is shown >T38179 GCTGCAGTTGGAGTATGAGATGGTCCTTACCAACGGCAAGTCTTTCTGGACACGGCACTTCTCAGCTGAT GAATTTGGGATTTTGGAGACAGATGTGACCTTTCTCCTCATCTTCACCCTTATCTTTGTCCTCTCTTGTT ACTTTGGATATTTGCTGAAAGGCCGTCAGTTACTCCACACAACTTATAAAATGTTCATGGCTGCAGCAGG AGTGGAGGTCCTGAGCCTCCTGTTTTTCTGCATCTACTGGGGCCAGTATGCCACAGATGGCATTGGCAAT GGCAGCGTGAAGATCTTGGCCAAGCTGCTCTTCTCCTCCAGTTTCCTCATCTTCCTGCTCACACTCATCC TCCTCGGGAAGGGATTCACGGTGACACGAGGCCGCATCAGCCACTCAGGCTCCGTGAAGTTGTCTGTGTA CATGACCCTGTACACCCTCACCCACGTGGTGTTGCTCATCTACGAGGCAGAATTCTTTGACCCAGGCCAG GTACTATACACATACGAGTCTCCGGCTGGTTATGGGCTCATCGGGCTGCAGGTGGCTGCCTATGTGTGGT TCTGCTATGCTGTGCTGGTCTCCCTTCGGCACTATCCAGAGAAGCAGCCCTTTTATGTGCCCTTCTTTGC TGCCTATACTCTCTGGTTCTTCGCTGTCCCCGTCATGGCTCTGATCGCCAATTTTGGGATCCCAAAGTGG GCCCGAGAGAAGATAGTCAACGGTATCCAGCTGGGGATCCACCTCTATGCCCATGGCGTGTTTCTGATCA TGACCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105762. Forward Primer - name:105762_F_cDNA_B930076A02, sequence:GCTGCAGTTGGAGTATGAGATG; Reverse Primer - name:105762_N_SP6_cDNA_B930076A02, sequence:GGGTCATGATCAGAAACACGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7881 same embryo
	EMAGE:7877 same embryo
	EMAGE:7879 same embryo
	EMAGE:7880 same embryo
	EMAGE:7876 same embryo
	EurExpress:euxassay_012829 same experiment
	MGI:4828776 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source