EMAGE mouse gene expression spatial database

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EMAGE:8152

Jdp2 Jun dimerization protein 2 ( MGI:1932093)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016441. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016441_01	euxassay_016441_02	euxassay_016441_03	euxassay_016441_04

euxassay_016441_05	euxassay_016441_06	euxassay_016441_07	euxassay_016441_08	euxassay_016441_09

euxassay_016441_10	euxassay_016441_11	euxassay_016441_12	euxassay_016441_13	euxassay_016441_14

euxassay_016441_15	euxassay_016441_16	euxassay_016441_17	euxassay_016441_18	euxassay_016441_19

euxassay_016441_20	euxassay_016441_21	euxassay_016441_22	euxassay_016441_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8152_wholemount_strong.wlz

8152_wholemount_moderate.wlz

8152_wholemount_weak.wlz

8152_wholemount_possible.wlz

8152_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8152_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

strong

spotted

strong expression: see section 01 18 22

forelimb digit 1 phalanx

strong

regional

strong expression: see section 01 moderate expression: see section 19

forelimb digit 2 phalanx

strong

regional

strong expression: see section 01 moderate expression: see section 18 19

forelimb digit 3 phalanx

moderate

regional

moderate expression: see section 20

hindlimb digit 1 phalanx

strong

regional

strong expression: see section 03 04 15 moderate expression: see section 16

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 04 05 weak expression: see section 13 14 15 16

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 04 05 13 14 weak expression: see section 15 16

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 04 05 14 weak expression: see section 13 15 16

hindlimb digit 5 phalanx

strong

regional

strong expression: see section 03 04 15 weak expression: see section 14 16

femur

strong

spotted

strong expression: see section 01 02 03 18 19

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 17

cerebral cortex mantle layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 17 18 19 20 21 22

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

olfactory cortex mantle layer

weak

regional

weak expression: see section 07 08 09 12 13 14

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 17

rest of cerebellum mantle layer

weak

regional

weak expression: see section 05 06 07 08 09 10 11 14 15 16 17 18

pons mantle layer

weak

regional

weak expression: see section 07 17

midbrain mantle layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 19 weak expression: see section 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 16 17 18 19 weak expression: see section 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 17

ventral grey horn

weak

regional

weak expression: see section 09 10 11 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 17 weak expression: see section 13 16 18

neural retina

weak

regional

weak expression: see section 01 21

mandible

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21

maxilla

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 12 13 14 15 16 17 18 19

scapula

strong

spotted

strong expression: see section 02 22 moderate expression: see section 21

axial skeleton tail region

moderate

regional

moderate expression: see section 08 09 weak expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63171
Entity Detected:	Jdp2, Jun dimerization protein 2 ( MGI:1932093)
Sequence:	sense strand is shown >T63171 AGAGGAGCTGAAATACGCTGACATCCGCAACATTGGGGCGATGATTGCGCCCTTGCACTTCCTGGAGGTG AAACTGGGCAAGAGGCCCCAACCCGTGAAGAGTGAGCTAGACGAGGAAGAAGAGCGAAGGAAAAGGCGCC GGGAAAAGAACAAAGTCGCTGCAGCCAGATGCCGGAACAAGAAGAAGGAACGCACAGAGTTTCTGCAGAG GGAGTCAGAGCGGCTGGAGCTCATGAACGCAGAGCTGAAGACGCAGATAGAGGAGCTGAAGCTGGAGCGG CAACAGCTTATCCTGATGCTCAACCGCCACCGCCCCACCTGCATCGTGCGCACAGATAGCGTCAGGACGC CCGAGTCCGAAGGCAACCCACTGCTGGAGCAGCTGGACAAGAAGTGACTGAAGGCCTGGAGGAGGCATCA GAGGAAGAGGAGGAAGGGGAGGAGCATAAAAGAGAAAGAGGACGAGCAAGGTGACAGAGGGCCCCTCCCA GGCACGTGACAAACTCTATGATGAGGCTTAGCATAACTAGCCTCCAGCTGGCTCTTTTTTGAAACTCAGC CCTGCCGCGCAAGAGCAAGAGCGGACTGAAGAAACCAGAGGGACCAGGTGCTGAGACCAAGGTTGACCCG CAGATAGGGGCTGTCCCACTCCAGGGCCCAGCTTGAAGAGCACCTGAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 143250. Forward Primer - name:143250_F_cDNA_Jundm2, sequence:AGAGGAGCTGAAATACGCTGAC; Reverse Primer - name:143250_N_SP6_cDNA_Jundm2, sequence:GCTCAGGTGCTCTTCAAGCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8156 same embryo
	EMAGE:8153 same embryo
	EMAGE:8154 same embryo
	EMAGE:8155 same embryo
	EurExpress:euxassay_016441 same experiment
	MGI:4825679 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source