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EMAGE:824

Otx2 orthodenticle homolog 2 (Drosophila) ( MGI:97451)
TS15 (9.7 dpc)
in situ hybridisation

Data Images
EMAGE:824 EMAGE:824
Fig 3A Martinez-Barbera et al, 2001 [PMID:11731459] . Copyright: This image is from Development and is displayed with the permission of the Comapny of Biologists Ltd who owns the copyright. Fig 3A' Martinez-Barbera et al, 2001 [PMID:11731459] . Copyright: This image is from Development and is displayed with the permission of the Comapny of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Dark (A) and bright-field (A') illuminations of the same section are shown. Image annotations: MHB - midbrain hinbrain boundary, hb - hindbrain, mb - midbrain, fb - forebrain.
Expression Pattern Description
Spatial Annotation:
EMAGE:824EMAGE:824Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
824_voxel_strong_3D_1.wlz
824_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:824_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalThe authors do not discuss this specimen in detail
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1309443
Entity Detected:Otx2, orthodenticle homolog 2 (Drosophila) ( MGI:97451)
Sequence:sense strand is shown

>MGI:1309443
AAATCTACCCACCAAGGACCCTGACCCTGTCCACTCCAGGCGAATCGAGACCGTCCGGCTGGGTCCCCCC
AATTTGGGCCGACTTTGCGCCTCCAAACAACCTTAGCATGATGTCTTATCTAAAGCAACCGCCTTACGCA
GTCAATGGGCTGAGTCTGACCACTTCGGGTATGGACTTGCTGCATCCCTCCGTGGGCTACCCCGCCACCC
CCCGGAAACAGCGAAGGGAGAGGACGACATTTACTAGGGCACAGCTCGACGTTCTGGAAGCTCTGTTTGC
CAAGACCCGGTACCCAGA
nt 226 - nt 523 of NM_144841.1
Notes:The Otx2 probe used in this study by Martinez-Barbera et al, 2001 [PMID:11731459] is defined as that described by Acampora et al, 1998 [PMID:9811592] ie. "Transcripts from the Otx2 wild-type allele were revealed by using the probe B (Fig. 1A) which includes 107 bp and 190 bp upstream and downstream the AUG, respectively".
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:(C57BL/6 x DBA/2)F1
Age:9.7 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Martinez-Barbera et al, 2001 [PMID:11731459] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11731459] Martinez-Barbera JP, Signore M, Boyl PP, Puelles E, Acampora D, Gogoi R, Schubert F, Lumsden A, Simeone A 2001 Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2. Development (128):4789-800
 [ PMID:9811592] Acampora D, Avantaggiato V, Tuorto F, Briata P, Corte G, Simeone A 1998 Visceral endoderm-restricted translation of Otx1 mediates recovery of Otx2 requirements for specification of anterior neural plate and normal gastrulation. Development (125):5091-104
Links:MGI:2155820 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI