EMAGE mouse gene expression spatial database

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EMAGE:8264

1110059M19Rik RIKEN cDNA 1110059M19 gene ( MGI:1916050)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007552. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007552_01	euxassay_007552_02	euxassay_007552_03	euxassay_007552_04

euxassay_007552_05	euxassay_007552_06	euxassay_007552_07	euxassay_007552_08	euxassay_007552_09

euxassay_007552_10	euxassay_007552_11	euxassay_007552_12	euxassay_007552_13	euxassay_007552_14

euxassay_007552_15	euxassay_007552_16	euxassay_007552_17	euxassay_007552_18	euxassay_007552_19

euxassay_007552_20	euxassay_007552_21	euxassay_007552_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8264_wholemount_strong.wlz

8264_wholemount_moderate.wlz

8264_wholemount_weak.wlz

8264_wholemount_possible.wlz

8264_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8264_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 22

upper leg muscle

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hand

moderate

regional

moderate expression: see section 21 22

foot

moderate

regional

moderate expression: see section 05 06 07 21

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 03 04 21 22

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon roof plate

strong

regional

strong expression: see section 12

choroid invagination

strong

regional

strong expression: see section 06 07 08 09 10 13 14 15 16 17 18

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

tongue muscle

moderate

regional

moderate expression: see section 07 08 09 10 11 12

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35350
Entity Detected:	1110059M19Rik, RIKEN cDNA 1110059M19 gene ( MGI:1916050)
Sequence:	sense strand is shown >T35350 GAGGCAGAAGCAAAGATCACTTCTCCAGACTACTCGGCTGGTGCTGGTGTTCAGCTGGTTTGCTCTTCCA TCTCATGAAAGTAATCTTTATCCTATTTCTACATGGCTTGTACTGAAAATGGGCTTGCAGGGCATAGCCA TTCACCCATAATAGTTCCTGTGGATAAAAACAGAAGCAAGGAGACATGCCACAATGTGCAGCTCCGCTCT CTGAGTTCCATGTTGAAGGATGATGAGGATGATGCAGATGTCTGGACCCGCCCTCCTGTCTCCTTGAGAC CTCCCTTCAATGTGCCAAGAACAGGAGCCCGCATTCCTCAAAATCCAAGAGCTCCAAGACACCCTCTTAC ACTAACACCTGCTATTGAGGAAGAGTCCCTAGCAACAGCAGAAATAAACAGCTCAGAGGGCCTGGAGAGC CAGAGCCAGAAGGGACATGATTCTATTAATATGTCCCAAGAATTCTCTGGAAGCCCTATGGCACTGATGA TCGGAGGCCCAAGAGTTGGTAGCAGGGTTCTTGAGAGAAGTGGAAATAACTCAAAGCCATATATTCCTGT GCCTAGATCCCAGGGCTTCTTTCCACCAAGGGGTTCACAGTCACGAGGTCCTCCATATATCCCTACGCTT AGATCAGGGATAATGATGGAAGTGACTCCAGGAAATGCTAGAATGGCAAACAGAGGCAACATGGCTCATG TTTCTTTCCCACTGGGTAGCCCAAGGCACCCTATGGATAATTGGCAACAGAGTCCTTCTCTCCCTTTGTC ACCCAGTATTACAGGCTTACCTTGCTCTTCTGCTCATTGCTTTCTGCCTCCCCAAGCTCCAGCATTCAAT CCATTTCCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86401. Forward Primer - name:086401_F_cDNA_1110059M19Rik, sequence:GAGGCAGAAGCAAAGATCACTT; Reverse Primer - name:086401_N_SP6_cDNA_1110059M19Rik, sequence:CAGGAAATGGATTGAATGCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8263 same embryo
	EMAGE:8262 same embryo
	EurExpress:euxassay_007552 same experiment
	MGI:4822599 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source