EMAGE mouse gene expression spatial database

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EMAGE:8331

Pacrg PARK2 co-regulated ( MGI:1916560)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007681. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007681_01	euxassay_007681_02	euxassay_007681_03	euxassay_007681_04

euxassay_007681_05	euxassay_007681_06	euxassay_007681_07	euxassay_007681_08	euxassay_007681_09

euxassay_007681_10	euxassay_007681_11	euxassay_007681_12	euxassay_007681_13	euxassay_007681_14

euxassay_007681_15	euxassay_007681_16	euxassay_007681_17	euxassay_007681_18	euxassay_007681_19

euxassay_007681_20	euxassay_007681_21	euxassay_007681_22	euxassay_007681_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8331_wholemount_strong.wlz

8331_wholemount_moderate.wlz

8331_wholemount_weak.wlz

8331_wholemount_possible.wlz

8331_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8331_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 06 07 08 09 10 11 14 15 16 18 19 weak expression: see section 17

4th ventricle

moderate

regional

moderate expression: see section 05 06 07 09 10 11 13 14 weak expression: see section 03 04 18 19 20

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 15

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 12 14 weak expression: see section 10 11

pons ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14

midbrain ventricular layer

moderate

regional

moderate expression: see section 13 weak expression: see section 09 10 11 12 14 15

spinal cord ventricular layer

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36734
Entity Detected:	Pacrg, PARK2 co-regulated ( MGI:1916560)
Sequence:	sense strand is shown >T36734 CTAAACTGCTGCCACAACAGACGTTCCAGGTGCACCAGCCTCGTTCCCTGGTTTCTGAGGGCTTCACAGT CAAAGCCATGATGAAAAACTCAGTCGTGCGGGGTCCTCCAGTTGCAGGGGCATTTAAAGAAAGGCCGGCC AAACCCACCACTTTCCGAAAATGTTATGAGCGAGGAGACTTCCCAATCGCCCTTGAGCATGACTCGAAAG GAAACAAAATTGCCTGGAAGGTTGAGATTGAGAAGTTGGACTACCATCATTACCTGCCTCTGTTTTTTGA TGGGCTTTCTGAAATGACGTTTCCCTATGAGTTTTTTGCTCGGCGAGGAATCCACGACATGCTGGAACAC GGAGGGAACAAGATTCTACCTGTCATTCCTCAGCTCATTATCCCAATAAAAAATGCCTTGAACCTCCGAA ACAGACAGATCATCTGTGTGACGCTCAAGGTTCTCCAGCATCTGGTTGTGTCTTCGGAGATGGTGGGCGA AGCTTTGTTGCCCTACTACCGTCAGATCCTGCCAATCCTGAACATCTTTAAGAACATGAATGTGAACTCC GGGGATGGCATCGACTACAGCCAGCAGAAGAGGGAGAACATTGGCGACCTGATCCAGGAGACCCTAGAAG CCTTCGAGCGCTACGGAGGGGAGGACGCCTTCATCAACATCAAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85151. Forward Primer - name:085151_F_cDNA_Pacrg, sequence:CTAAACTGCTGCCACAACAGAC; Reverse Primer - name:085151_N_SP6_cDNA_Pacrg, sequence:TTTGATGTTGATGAAGGCGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8334 same embryo
	EMAGE:8333 same embryo
	EMAGE:8332 same embryo
	EurExpress:euxassay_007681 same experiment
	MGI:4827020 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source