EMAGE mouse gene expression spatial database

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EMAGE:8373

Trp73 transformation related protein 73 ( MGI:1336991)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009505. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009505_01	euxassay_009505_02	euxassay_009505_03	euxassay_009505_04

euxassay_009505_05	euxassay_009505_06	euxassay_009505_07	euxassay_009505_08	euxassay_009505_09

euxassay_009505_10	euxassay_009505_11	euxassay_009505_12	euxassay_009505_13	euxassay_009505_14

euxassay_009505_15	euxassay_009505_16	euxassay_009505_17	euxassay_009505_18	euxassay_009505_19

euxassay_009505_20	euxassay_009505_21	euxassay_009505_22	euxassay_009505_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8373_wholemount_strong.wlz

8373_wholemount_moderate.wlz

8373_wholemount_weak.wlz

8373_wholemount_possible.wlz

8373_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8373_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 06 09 10 16 17 18 19 20

vibrissa

moderate

regional

moderate expression: see section 02 03 04 05 06 16 17 18 19 20 21 23

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 08 09 10 11 14 15 16

choroid invagination

moderate

regional

moderate expression: see section 05 06 07 14 15 16 17 18 19

telencephalon mantle layer

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 13 14 15 16 17 18 19 weak expression: see section 12

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 12

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 03 04 05 06 09 10 16 17 18 19 20 21

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 14 15 16 moderate expression: see section 13

vomeronasal organ

strong

regional

strong expression: see section 10 moderate expression: see section 13

lower jaw incisor

moderate

regional

moderate expression: see section 08 09 10 14 weak expression: see section 12 13

lower jaw molar

moderate

regional

moderate expression: see section 05 06 16 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 09 10 14 weak expression: see section 13

upper jaw molar

moderate

regional

moderate expression: see section 05 06 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36872
Entity Detected:	Trp73, transformation related protein 73 ( MGI:1336991)
Sequence:	sense strand is shown >T36872 CCTTTGGTTGACTCCTATCGACAGCAGCAGCAGCAGCAGCTCCTACAGAGGCCGAGTCACCTGCAGCCTC CATCCTATGGGCCCGTGCTCTCCCCAATGAACAAGGTACACGGTGGTGTCAACAAACTGCCCTCCGTCAA CCAGCTGGTGGGCCAGCCTCCCCCGCACAGCTCAGCAGCTGGGCCCAACCTGGGGCCCATGGGCTCCGGG ATGCTCAACAGCCACGGCCACAGCATGCCGGCCAATGGTGAGATGAATGGAGGCCACAGCTCCCAGACCA TGGTTTCGGGATCCCACTGCACCCCGCCACCCCCCTATCATGCAGACCCCAGCCTCGTCAGTTTTTTGAC AGGGTTGGGGTGTCCAAACTGCATCGAGTGCTTCACTTCCCAAGGGTTGCAGAGCATCTACCACCTGCAG AACCTTACCATCGAGGACCTTGGGGCTCTGAAGGTCCCTGACCAGTACCGTATGACCATCTGGAGGGGCC TACAGGACCTGAAGCAGAGCCATGACTGCGGCCAGCAACTGCTACGCTCCAGCAGCAACGCGGCCACCAT CTCCATCGGCGGCTCTGGCGAGCTGCAGCGGCAGCGGGTCATGGAAGCCGTGCATTTCCGTGTGCGCCAC ACCATCACAATCCCCAACCGTGGAGGCGCAGGTGCGGTGACAGGTCCCGACGAGTGGGCGGACTTTGGCT TTGACCTGCCTGACTGCAAGTCCCGTAAGCAGCCCATCAAAGAGGAGTTCACAGAGACAGAGAGCCACTG AGGAACGTAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90471. Forward Primer - name:090471_F_cDNA_Trp73, sequence:CCTTTGGTTGACTCCTATCGAC; Reverse Primer - name:090471_N_SP6_cDNA_Trp73, sequence:GTACGTTCCTCAGTGGCTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8370 same embryo
	EMAGE:8371 same embryo
	EMAGE:8374 same embryo
	EMAGE:8372 same embryo
	EMAGE:8369 same embryo
	EurExpress:euxassay_009505 same experiment
	MGI:4828936 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source