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EMAGE:8652

2310003H01Rik RIKEN cDNA 2310003H01 gene ( MGI:1919135)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8652 EMAGE:8652 EMAGE:8652 EMAGE:8652 EMAGE:8652
"Pseudo-wholemount" of euxassay_009994. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009994_01 euxassay_009994_02 euxassay_009994_03 euxassay_009994_04
EMAGE:8652 EMAGE:8652 EMAGE:8652 EMAGE:8652 EMAGE:8652
euxassay_009994_05 euxassay_009994_06 euxassay_009994_07 euxassay_009994_08 euxassay_009994_09
EMAGE:8652 EMAGE:8652 EMAGE:8652 EMAGE:8652 EMAGE:8652
euxassay_009994_10 euxassay_009994_11 euxassay_009994_12 euxassay_009994_13 euxassay_009994_14
EMAGE:8652 EMAGE:8652 EMAGE:8652 EMAGE:8652 EMAGE:8652
euxassay_009994_15 euxassay_009994_16 euxassay_009994_17 euxassay_009994_18 euxassay_009994_19
EMAGE:8652 EMAGE:8652 EMAGE:8652
euxassay_009994_20 euxassay_009994_21 euxassay_009994_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8652Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8652_wholemount_strong.wlz
8652_wholemount_moderate.wlz
8652_wholemount_weak.wlz
8652_wholemount_possible.wlz
8652_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8652_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 18 19 20 21 22
humerus
moderate moderate
regionalmoderate expression: see section 01
femur
moderate moderate
regionalmoderate expression: see section 03 04 05 20 21 22
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 19 20
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 19
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 17 18 19 20
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 08
spinal cord
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 16 17 18
mandible
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 13 14 15 16 17 18 19
maxilla
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 13 14 15 16 17 18
bladder
moderate moderate
regionalmoderate expression: see section 12 13 14 15
cranium
moderate moderate
regionalmoderate expression: see section 01 02
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 02 03 18 19 20 21
clavicle
strong strong
regionalstrong expression: see section 06 07 08 09 17 18 moderate expression: see section 19 20
scapula
moderate moderate
regionalmoderate expression: see section 02 03 04 05 21 22
tail paraxial mesenchyme
moderate moderate
regionalmoderate expression: see section 09 10 11 13 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31751
Entity Detected:2310003H01Rik, RIKEN cDNA 2310003H01 gene ( MGI:1919135)
Sequence:sense strand is shown

>T31751
CCATGTCCTGCTGGCTCTTCTACAGCCTCAGGGAGGTGGTGGGTGCGGCCCTGGCCCCCTCAGACCCTCT
AGAGGCCCCCTACTTGGAGCAGTTCCCTCTCAGCCTTCCTAAGCAAGAGGGCGTCTGCCTGCCCCTGTGC
AAGCGCACAGTGGACATGCTGCAGTGCCTGCGCTTCGCCGGCGCAGCCACACACCCTGCACAGGCCCCCT
GTATGCCTGGCCCTGCCTGTGAGCCTGTGGAAACTTTCCTGAAGACCTGCCAAGCCCCTGGCAGCGAGCC
TACTGGCGCTGCCTCCCTACGGGCCAAGTACCTGCCTCCATCCACAGCCTCCATCAGGGTGTCTGCAGGC
CTTCTCAGAGCTGCCCTGGAAGACAGTCACTCAGGTTTCCACCTGTGCAGCGCCACCCTGCGGTGGCTCC
TTGCCGAGAACGCTGCTGTGGATGTTGTGCGGGCCCAGACACTGTCTTCCATCCAGGGAATAGCCCCAGA
TGGAACTGATGTCAACCTCACCGTCCATGAGGTAGCTGTGACGGATTTGAGCCCTGCAGGCCCCATTCAG
GCTGTGGAGATCCAAGTGGAGAGCTCCTCTCTGGCCAACATGTGTAGGGCGCACCATGCCATCATCAGAC
GAATACAGACAATGGTCACAGAGCAAGCAGCCCTAGGCTCCAGCCCACCTGACCTCCGCATGCAGTATCT
ACAGCAGATCCATGCCAACCACCAGGAACTGCTCCGTGAGGTGCAAGCACTGCGCGACCAGCTGTGCACA
GAGGATGAGCTGAGCTCCTGCTCCACGGCCCAGAAGCTCCTGCACATTTACAAGCAGCTGCGCAACCCTA
GCCTCGTCCTGTTGTGACCAGCTGCTGGCCGGCTCCACGTCACTGCAGGACACACTTGGGGCTTGCCTGG
CAGCATTCCTCAACTGGAACCTCTGCACCCTGATGGGAAATACCGTCACCCAAGAACTCAGGCCATGGAG
ACACAGGGTTTCTGGGGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6399141), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58297. Forward Primer - name:058297_F_IRAV92_f05_2310003H01Rik, sequence:CCATGTCCTGCTGGCTCT; Reverse Primer - name:058297_R_SP6_IRAV92_f05_2310003H01Rik, sequence:GAGCCCCAGAAACCCTGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8651 same embryo
 EMAGE:8649 same embryo
 EMAGE:8653 same embryo
 EMAGE:8648 same embryo
 EMAGE:8650 same embryo
 EurExpress:euxassay_009994 same experiment
 MGI:4822658 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS