EMAGE mouse gene expression spatial database

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EMAGE:8770

Bex2 brain expressed X-linked 2 ( MGI:1338017)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009948. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009948_01	euxassay_009948_02	euxassay_009948_03	euxassay_009948_04

euxassay_009948_05	euxassay_009948_06	euxassay_009948_07	euxassay_009948_08	euxassay_009948_09

euxassay_009948_10	euxassay_009948_11	euxassay_009948_12	euxassay_009948_13	euxassay_009948_14

euxassay_009948_15	euxassay_009948_16	euxassay_009948_17	euxassay_009948_18	euxassay_009948_19

euxassay_009948_20	euxassay_009948_21	euxassay_009948_22	euxassay_009948_23	euxassay_009948_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8770_wholemount_strong.wlz

8770_wholemount_moderate.wlz

8770_wholemount_weak.wlz

8770_wholemount_possible.wlz

8770_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8770_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22 23 24

upper leg muscle

moderate

regional

moderate expression: see section 01 02 17 18 19 21 weak expression: see section 03 04 15 16

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 21

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pancreas

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 19 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 18 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 08 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 18 19

trigeminal v nerve

strong

regional

strong expression: see section 09 17

spinal cord

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 13 14 15 16

cervical ganglion

strong

regional

strong expression: see section 08 09 16 17

thoracic ganglion

strong

regional

strong expression: see section 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

neural retina

strong

regional

strong expression: see section 01 02 03 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 17 moderate expression: see section 08 09 10 11 12 14 15 16 18 19

vomeronasal organ

moderate

regional

moderate expression: see section 12 15

tongue muscle

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 10 11 15

stomach

moderate

regional

moderate expression: see section 06 07 08 09

midgut

strong

regional

strong expression: see section 13 14 21 22 23 24 moderate expression: see section 12 15 16 17 18 19 20

liver

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 01 02 03 04 20 21 22 23 24

bladder

strong

regional

strong expression: see section 10 11 12

metanephros

moderate

regional

moderate expression: see section 07 13 14 weak expression: see section 05 06 15

seminiferous cord

strong

spotted

strong expression: see section 05 06 16 17 18 moderate expression: see section 03 04

left lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10

right lung

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 06 07 13 14 15 16 weak expression: see section 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31117
Entity Detected:	Bex2, brain expressed X-linked 2 ( MGI:1338017)
Sequence:	sense strand is shown >T31117 CGAGCGGGACAGATTGACTGGAAACCGAGAGTCCAGGCGGCAGCGGGAATTGACAGGAGGACTACGCCGC AAGGGATAGGCCCAGGAGAAATGGAGTCCAAAGTGGAACAAGGTGTGAAAAATCTCAACATGGAGAATGA CCATCAGGAAAAGGAGGAAAAGGAAGAAAAGCCACAGGATGCTAGCAAAAGGGATCCGATTGTGGCCCTG CCTTTCGAAGCTGGAGACTACTACGTGCCTAGAGGAGGTCGCAGGCGGTTCCGGGTTCGGCAGCCCATCG TGCACTACAGATGGGACCTGATGCATAGGGTTGGGGAGCCCCAGGGAAGGATGAGAGAGGAGAACGTACA GAGGTTTGGGGATGATGTGAGACAGCTCATGGAGAAGCTGAGGGAAAGGCAGCTGAGCCACAGCCTGCGG GCGGTTAGCACTGACCCGCCTCATCATGACCACCATGATGAGTTTTGCCTTATGCCCTGAGTCCTGAGCT TTTCTCTGAAGATGACATGGGGACCCCTGCTTTCAAAACTTACATGTTTGTGATGTACTGTTGTAATATT TCTGGTGTCACTTGTTTCCCCATGGGGCTCCAACTTCTGCCAGCTTCTAATTGAAACTTGTGTTTTCGAC TCAGTCTTACATTTCTTTTTTTGTTAGCAGAATTTATCTATTGCATGGGAAGATGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5058536), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 56576. Forward Primer - name:056576_F_IRAV43_c05_Bex2, sequence:CGAGCGGGACAGATTGAC; Reverse Primer - name:056576_R_SP6_IRAV43_c05_Bex2, sequence:GAGCATCTTCCCATGCAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8771 same embryo
	EMAGE:8774 same embryo
	EMAGE:8773 same embryo
	EMAGE:8775 same embryo
	EMAGE:8772 same embryo
	EurExpress:euxassay_009948 same experiment
	MGI:4823460 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source