EMAGE mouse gene expression spatial database

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EMAGE:8814

Ptp4a1 protein tyrosine phosphatase 4a1 ( MGI:1277096)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010021. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010021_01	euxassay_010021_02	euxassay_010021_03	euxassay_010021_04

euxassay_010021_05	euxassay_010021_06	euxassay_010021_07	euxassay_010021_08	euxassay_010021_09

euxassay_010021_10	euxassay_010021_11	euxassay_010021_12	euxassay_010021_13	euxassay_010021_14

euxassay_010021_15	euxassay_010021_16	euxassay_010021_17	euxassay_010021_18	euxassay_010021_19

euxassay_010021_20	euxassay_010021_21	euxassay_010021_22	euxassay_010021_23	euxassay_010021_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8814_wholemount_strong.wlz

8814_wholemount_moderate.wlz

8814_wholemount_weak.wlz

8814_wholemount_possible.wlz

8814_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8814_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 06 07 weak expression: see section 05 14 15

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 18 19

forebrain

moderate

regional

moderate expression: see section 21 22 23 24 weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

hindbrain

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord

weak

regional

weak expression: see section 07 08 09 10 11 12 13

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 15

cervical ganglion

weak

regional

weak expression: see section 08 16

thoracic ganglion

weak

regional

weak expression: see section 10 11

neural retina

weak

regional

weak expression: see section 02 03 04 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 16 weak expression: see section 09 14 15 17 18

stomach

moderate

regional

moderate expression: see section 05 06 07 08 weak expression: see section 09

lower jaw incisor

weak

regional

weak expression: see section 11 12 15 16

upper jaw incisor

weak

regional

weak expression: see section 11 12 15 16

bladder

weak

regional

weak expression: see section 11 12 13 14

lung

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31760
Entity Detected:	Ptp4a1, protein tyrosine phosphatase 4a1 ( MGI:1277096)
Sequence:	sense strand is shown >T31760 TCCAACCAATGCGACCTTAAACAAATTTATAGAGGAACTTAAGAAGTATGGAGTTACCACAATAGTAAGA GTATGTGAAGCAACTTACGACACTACTCTTGTGGAGAAAGAAGGCATTCATGTTCTTGACTGGCCTTTTG ATGATGGTGCACCACCATCCAACCAGATTGTCGATGACTGGCTAAGTCTTGTGAAGATTAAGTTTCGTGA AGAACCTGGTTGCTGTATTGCTGTCCATTGTGTCGCAGGCCTTGGCAGAGCTCCGGTGCTTGTTGCCCTA GCATTAATTGAAGGTGGAATGAAATATGAAGATGCAGTACAATTCATAAGACAAAAGCGGCGTGGAGCTT TTAACAGCAAGCAACTTTTGTATCTGGAGAAGTACCGTCCGAAAATGCGGCTCCGCTTCAAGGATTCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6396041), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60678. Forward Primer - name:060678_F_IRAV95_a11_Ptp4a1, sequence:TCCAACCAATGCGACCTT; Reverse Primer - name:060678_R_SP6_IRAV95_a11_Ptp4a1, sequence:TTGGAATCCTTGAAGCGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8812 same embryo
	EMAGE:8811 same embryo
	EMAGE:8813 same embryo
	EMAGE:8810 same embryo
	EurExpress:euxassay_010021 same experiment
	MGI:4827513 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source