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EMAGE:8823

Serpinb6a serine (or cysteine) peptidase inhibitor, clade B, member 6a ( MGI:103123)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823
"Pseudo-wholemount" of euxassay_010066. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010066_01 euxassay_010066_02 euxassay_010066_03 euxassay_010066_04
EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823
euxassay_010066_05 euxassay_010066_06 euxassay_010066_07 euxassay_010066_08 euxassay_010066_09
EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823
euxassay_010066_10 euxassay_010066_11 euxassay_010066_12 euxassay_010066_13 euxassay_010066_14
EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823
euxassay_010066_15 euxassay_010066_16 euxassay_010066_17 euxassay_010066_18 euxassay_010066_19
EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823 EMAGE:8823
euxassay_010066_20 euxassay_010066_21 euxassay_010066_22 euxassay_010066_23 euxassay_010066_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8823Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8823_wholemount_strong.wlz
8823_wholemount_moderate.wlz
8823_wholemount_weak.wlz
8823_wholemount_possible.wlz
8823_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8823_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
diaphragm
weak weak
regionalweak expression: see section 07 08 09 10 11 12 13 16 17 18 19 20 21
mesenchyme
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
adrenal gland
strong strong
regionalstrong expression: see section 07 08 09 15 16 17 weak expression: see section 18
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 18 19 20 21
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 10 11 17 18
pons mantle layer
weak weak
regionalweak expression: see section 09 19
facial vii ganglion
weak weak
regionalweak expression: see section 06 07 22 23
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 09 moderate expression: see section 08 20 weak expression: see section 21
trigeminal v ganglion
weak weak
regionalweak expression: see section 05 06 07 08 09 10 18 19 20 21 22 23 24
vagus x ganglion
strong strong
regionalstrong expression: see section 10 19
ventral grey horn
weak weak
regionalweak expression: see section 11 12 14 15 16
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 10 11 14 15 16 17 18 19 20
hindgut
moderate moderate
regionalmoderate expression: see section 13 14
rectum
moderate moderate
regionalmoderate expression: see section 12 13
midgut
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18 19 20
testis
strong strong
regionalstrong expression: see section 05 06 07 08 18 19 20 21
trachea
weak weak
regionalweak expression: see section 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31809
Entity Detected:Serpinb6a, serine (or cysteine) peptidase inhibitor, clade B, member 6a ( MGI:103123)
Sequence:sense strand is shown

>T31809
GGGGCAAAAGGAACCACTGCTAGCCAGATGGCTCAGGCACTTGCTTTGGATAAATGCAGCGGCAATGGAG
GTGGAGATGTCCACCAGGGCTTCCAGTCCCTTCTCACCGAAGTGAACAAAACTGGCACACAGTACTTGCT
CAGAACAGCCAACAGGCTCTTCGGGGATAAGACTTGTGATCTTTTAGCGTCTTTTAAAGATTCCTGCCTC
AAGTTCTATGAAGCAGAGTTGGAAGAGCTGGACTTTCAGGGTGCTACAGAGGAGTCCCGACAGCACATCA
ACACCTGGGTAGCCAAAAAGACAGAAGATAAAATCAAAGAGGTGCTGTCTCCAGGTACAGTGAATTCTGA
TACATCGCTAGTCCTTGTGAATGCCATCTACTTCAAAGGAAACTGGGAGAAGCAGTTTAACAAAGAGCAT
ACCAGGGAGATGCCATTCAAAGTCAGCAAGAATGAGGAGAAACCTGTGCAAATGATGTTTAAGAAGTCTA
CCTTTAAGATGACCTATATTGGAGAGATATTCACTAAGATTCTGTTGCTTCCCTATGTCAGCAGTGAGCT
GAACATGATCATCATGCTTCCAGATGAGCACGTTGAACTGAGTACAGTGGAAAAGGAAGTAACTTACGAG
AAATTTATAGAGTGGACAAGGCTGGACAAGATGGACGAAGAAGAGGTAGAAGTATTTCTCCCAAAGTTTA
AGCTGGAGGAGAATTACAACATGAACGATGCCCTCTACAAGTTGGGCATGACTGATGCCTTTGGCGGCAG
GGCAGACTTTTCTGGAATGTCTTCCAAGCAAGGCTTGTTTCTGTCTAAGGTTGTGCATAAGGCCTTTGTG
GAGGTTAATGAGGAGGGCACAGAGGCTGCAGCTGCTACAGCTGGCATGATGACGGTGAGGTGCATGAGAT
TCACTCCCCGCTTCTGTGCCGACCACCCCTTCCTTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5342439), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60284. Forward Primer - name:060284_F_IRAV98_g12_Serpinb6a, sequence:GGGGCAAAAGGAACCACT; Reverse Primer - name:060284_R_SP6_IRAV98_g12_Serpinb6a, sequence:AAAAGGAAGGGGTGGTCG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8825 same embryo
 EMAGE:8821 same embryo
 EMAGE:8826 same embryo
 EMAGE:8824 same embryo
 EMAGE:8822 same embryo
 EurExpress:euxassay_010066 same experiment
 MGI:4827993 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS