EMAGE mouse gene expression spatial database

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EMAGE:893

Lfng LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ( MGI:1095413)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Fig 6B Koizumi et al, 2001 [PMID:11262239] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.	Fig 6A Koizumi et al, 2001 [PMID:11262239] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.	Fig 6C Koizumi et al, 2001 [PMID:11262239] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

These sets of images show the dynamic cyclical expression pattern of Lfng in the presomitic mesoderm. The data from Image 6Bc was used for spatial input into this EMAGE entry as it covers all possible regions shown in the entire data set. Left hand image set (Fig 6B abcd) - Progression of wave front of cyclic expression of Lfng in the wild type embryos. Centre image set (Fig6A abcd) - To allow quantitative comparison, chromogenic reaction was limited to 30 mins. Under this condition, the dynamic expression of Lfng was clearly seen in the wild type embryo shown. Right hand image set (Fig 6C abc) - Comparison of Lfng expression between uncultured (left) and cultured (right) halves of tails with various times as shown.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

893_wholemount_strong_3D_1.wlz

893_wholemount_moderate_3D_1.wlz

893_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:893_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

tail unsegmented mesenchyme

strong

regional

This series of experiments illustrates an oscillation of expression of the gene in unsegmented mesoderm

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1276421
Entity Detected:	Lfng, LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ( MGI:1095413)
Notes:	The probe used in this study by Koizumi et al, 2001 [PMID:11262239] is indicated as that described by Evrard et al, 1998 [PMID:9690473] ie. "The entire insert from pMFR1". pMFR1 is 2.2kb long and was isolated by screening an 8.5-d.p.c. whole-embryo cDNA library (from B. Hogan) with a human expressed-sequence tag (EST). Exact sequence of this clone is not reported.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Koizumi et al, 2001 [PMID:11262239] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:11262239] Koizumi K, Nakajima M, Yuasa S, Saga Y, Sakai T, Kuriyama T, Shirasawa T, Koseki H 2001 The role of presenilin 1 during somite segmentation. Development (128):1391-402
	[ doi:10.1038/28632] [ PMID:9690473] Evrard YA, Lun Y, Aulehla A, Gan L, Johnson RL 1998 lunatic fringe is an essential mediator of somite segmentation and patterning. Nature (394):377-81
Links:	MGI:1932861 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source