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EMAGE:8936

Krt27 keratin 27 ( MGI:1339999)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8936 EMAGE:8936 EMAGE:8936 EMAGE:8936 EMAGE:8936
"Pseudo-wholemount" of euxassay_012869. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012869_01 euxassay_012869_02 euxassay_012869_03 euxassay_012869_04
EMAGE:8936 EMAGE:8936 EMAGE:8936 EMAGE:8936 EMAGE:8936
euxassay_012869_05 euxassay_012869_06 euxassay_012869_07 euxassay_012869_08 euxassay_012869_09
EMAGE:8936 EMAGE:8936 EMAGE:8936 EMAGE:8936 EMAGE:8936
euxassay_012869_10 euxassay_012869_11 euxassay_012869_12 euxassay_012869_13 euxassay_012869_14
EMAGE:8936 EMAGE:8936 EMAGE:8936 EMAGE:8936 EMAGE:8936
euxassay_012869_15 euxassay_012869_16 euxassay_012869_17 euxassay_012869_18 euxassay_012869_19
EMAGE:8936 EMAGE:8936 EMAGE:8936 EMAGE:8936
euxassay_012869_20 euxassay_012869_21 euxassay_012869_22 euxassay_012869_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8936Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8936_wholemount_strong.wlz
8936_wholemount_moderate.wlz
8936_wholemount_weak.wlz
8936_wholemount_possible.wlz
8936_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8936_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 06 16 17 18 19 20 21 22
radius
moderate moderate
regionalmoderate expression: see section 23
humerus
moderate moderate
regionalmoderate expression: see section 20 21 22 23 weak expression: see section 01 02 19
fibula
moderate moderate
regionalmoderate expression: see section 23 weak expression: see section 01
tibia
moderate moderate
regionalmoderate expression: see section 22 23 weak expression: see section 01
femur
moderate moderate
regionalmoderate expression: see section 03 04 05 19 20 21 22 23 weak expression: see section 02 06 18
vibrissa
moderate moderate
regionalmoderate expression: see section 18 weak expression: see section 03 04 05 17
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 07 08 13 14 15
pons mantle layer
weak weak
regionalweak expression: see section 06 15 16
ventral grey horn
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 08 09 10 12 13 14 15
mandible
weak weak
regionalweak expression: see section 02 03 04 05 06 07 09 13 14 15 16 17 18
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 06 07 08 09 13 14 15 16 17 weak expression: see section 03 04 05 18
maxilla
weak weak
regionalweak expression: see section 03 04 16
basioccipital bone
weak weak
regionalweak expression: see section 08 09 10 11 12 13
basisphenoid bone
weak weak
regionalweak expression: see section 08 09 10 11 12
exoccipital bone
moderate moderate
regionalmoderate expression: see section 04 weak expression: see section 03 17 18 19 20
vault of skull
weak weak
regionalweak expression: see section 01 02 03 19 20 21 23
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 21 weak expression: see section 03 04 05 17 18 19 20 22
scapula
moderate moderate
regionalmoderate expression: see section 02 20 21
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 08 09 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38526
Entity Detected:Krt27, keratin 27 ( MGI:1339999)
Sequence:sense strand is shown

>T38526
GGCATACCTCAAGAAGAACCACGAGGAGGAAATGCAGGCTCTGCAGTGCGCCGCTGGAGGCAACGTGAAC
GTGGAGATGAACGCAGCCCCGGGCGTGGACCTCACCGTCCTGCTGAACAACATGAGAGCCGAGTACGAGG
CCCTGGCTGAGCAGAACCGTAGGGACGCCGAGGCCTGGTTCCAAGAGAAGAGCGCCTCCCTGCAGCAGCA
GATTTCAGACGATGCTGGAGCCACCACCTCAGCCAGGAACGAACTTACAGAGATGAAACGGACTCTCCAA
ACCCTGGAGATAGAGCTACAGTCCCTCTTAGCCATGAAACACTCTCTGGAGTGCTCCCTGACGGAGACCG
AGGGCAACTACTGCACGCAGCTGGCTCAGATCCAGGCTCAGATCAGCGCCCTGGAGGAGCAGCTGCACCA
AGTGAGGACGGAGACGGAGGGCCAGAAGCTGGAATATGAGCAGCTGCTGAATGTCAAGGCCCACCTGGAG
AAGGAGATCGAGACCTACTGCCGCCTCATAGATGGAGACGAGGGCTCTTGTGTTAAGGCAAAAGGCCAAG
GAAGACCCGGAAATCAGACAAAAGATTCGCCTAAAACTGCCATCGTTAAAACTGTCGTGGAAGAGTTAGA
CCCCCGTGGCAAGGTTCTCTCCTCCAGAGTTCACACCCTGGAAGAGAAATCCACCAAAGTCAACAAGACC
GAGCAGAGGATC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 150736. Forward Primer - name:150736_F_cDNA_Krt1-c29, sequence:GGCATACCTCAAGAAGAACCAC; Reverse Primer - name:150736_N_SP6_cDNA_Krt1-c29, sequence:GATCCTCTGCTCGGTCTTGTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8937 same embryo
 EMAGE:8938 same embryo
 EMAGE:8934 same embryo
 EMAGE:8935 same embryo
 EMAGE:8939 same embryo
 EurExpress:euxassay_012869 same experiment
 MGI:4825836 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS