EMAGE mouse gene expression spatial database

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EMAGE:8945

Zcchc3 zinc finger, CCHC domain containing 3 ( MGI:1915167)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012888. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012888_01	euxassay_012888_02	euxassay_012888_03	euxassay_012888_04

euxassay_012888_05	euxassay_012888_06	euxassay_012888_07	euxassay_012888_08	euxassay_012888_09

euxassay_012888_10	euxassay_012888_11	euxassay_012888_12	euxassay_012888_13	euxassay_012888_14

euxassay_012888_15	euxassay_012888_16	euxassay_012888_17	euxassay_012888_18	euxassay_012888_19

euxassay_012888_20	euxassay_012888_21	euxassay_012888_22	euxassay_012888_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8945_wholemount_strong.wlz

8945_wholemount_moderate.wlz

8945_wholemount_weak.wlz

8945_wholemount_possible.wlz

8945_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8945_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 04 05 17 18 19 20 21

humerus

moderate

regional

moderate expression: see section 02 03 weak expression: see section 04 21 22 23

femur

weak

regional

weak expression: see section 01 02 03 04 16 17 18 19 20

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 15

ventral grey horn

weak

regional

weak expression: see section 06 08 09 10 11 12 13

meckel's cartilage

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 14 15 16 17 18 19

axial skeleton

moderate

regional

moderate expression: see section 07 14 15 16 weak expression: see section 08 12 13

basioccipital bone

weak

regional

weak expression: see section 11 12 13 14 15

basisphenoid bone

weak

regional

weak expression: see section 11 12 13 14

exoccipital bone

weak

regional

weak expression: see section 04 06

temporal bone

weak

regional

weak expression: see section 03

vault of skull

weak

regional

weak expression: see section 01 02 03 04

orbito-sphenoid

weak

regional

weak expression: see section 01 02 03 04 06 07 08

scapula

moderate

regional

moderate expression: see section 02 03 weak expression: see section 04 20

pelvic girdle skeleton

moderate

regional

moderate expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38641
Entity Detected:	Zcchc3, zinc finger, CCHC domain containing 3 ( MGI:1915167)
Sequence:	sense strand is shown >T38641 GAAAAGCTAGCCCTGTTTCTCCGCGTCTACGAGGAGAAGCGAGAGCTGGAGGACTGCTGGGAGAACTTTG TGGTTTTGGGGCGGAGCAGGTCCAGCCTGAAGACCCTCTTCATCCTCTTCCGGAACGAGACCGTGGACGT GGAGGACATCGTGACCTGGCTCAAGCGCCACTGCGACGTGCTGGCCGTGCCCGTGAAAGTGACCGACAGG TTCGGGATCTGGACCGGGGAGTACAAGTGCGAGATCGAGCTGCGCCAGGGGGAGGGCGGAGTGAGGCACC TGCCCGGGGCCTTCTTCTTGGGAGCGGAGAGGGGCTACAGCTGGTACAAGGGGCAGCCCAAGACGTGCTT TAAGTGTGGTTCCCGGACCCACATGAGCGGCACCTGCACGCAGGACAGGTGTTTCAGGTGCGGGGAAGAG GGGCACCTGAGCCCTTACTGCCGGAAGGTCATCGTGTGCAACCTCTGTGGCAAAAGAGGACACGCCTTTG CCCAGTGTCCCAAAGCAGTTCACAATTCCGTGACAGCTCAGCTCACCAGCGTGGCGGGGCACTGAAGGCG CGACTGCCTGCCAGGGTGAATACACTGCCAGCTTAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 143490. Forward Primer - name:143490_F_cDNA_Zcchc3, sequence:GAAAAGCTAGCCCTGTTTCTCC; Reverse Primer - name:143490_N_SP6_cDNA_Zcchc3, sequence:GTAAGCTGGCAGTGTATTCACCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8940 same embryo
	EMAGE:8941 same embryo
	EMAGE:8942 same embryo
	EMAGE:8943 same embryo
	EMAGE:8944 same embryo
	EurExpress:euxassay_012888 same experiment
	MGI:4829274 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source