EMAGE mouse gene expression spatial database

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EMAGE:8961

Ywhab tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide ( MGI:1891917)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012917. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012917_01	euxassay_012917_02	euxassay_012917_03	euxassay_012917_04

euxassay_012917_05	euxassay_012917_06	euxassay_012917_07	euxassay_012917_08	euxassay_012917_09

euxassay_012917_10	euxassay_012917_11	euxassay_012917_12	euxassay_012917_13	euxassay_012917_14

euxassay_012917_15	euxassay_012917_16	euxassay_012917_17	euxassay_012917_18	euxassay_012917_19

euxassay_012917_20	euxassay_012917_21	euxassay_012917_22	euxassay_012917_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8961_wholemount_strong.wlz

8961_wholemount_moderate.wlz

8961_wholemount_weak.wlz

8961_wholemount_possible.wlz

8961_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8961_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 09 10 11 15

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 11 12 14 15

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 11 15 16

pons mantle layer

moderate

regional

moderate expression: see section 08 09 10 16 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 08 16

trigeminal v nerve

moderate

regional

moderate expression: see section 09

ventral grey horn

moderate

regional

moderate expression: see section 11 12 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38677
Entity Detected:	Ywhab, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide ( MGI:1891917)
Sequence:	sense strand is shown >T38677 TGTGCTCTGTGATCTGTTTGTGTCACTTTGTATCCTCCACATTATGTCCCTTTGTGCGATAAACAAAAAC AAAAAATCGAATGTGGACTTTCGATTTTTCACAGCCTAAGCCTTGCAAAGATGGTCCCTGGGATGAACAG CTGGTATTTGTATCTAAAGCTCAGACTGGTCCCTTAATACCATGGTATTGTGAAGTCTTGATTTTGATCA ACATTGACAAGGATTACTGTGTGTTTAATTTTTACAAACTGAACACTGTGATCATGGGGTTTTATAACTT AGCAGAACTCTTTCTGGTAGGAATAATAGACCTGAATTATGTGTAACTTCTTGGAAAGTTTAATCTGATA TCAAAATGGTCACTGAAATACAATTCTGTTGTAAAGCTGTACAGAAAGTTCTAGAGATTCTGTGGTGATG CTGGGACTTGGTGAGATGCTGACCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 155217. Forward Primer - name:155217_F_cDNA_TC1567445, sequence:TGTGCTCTGTGATCTGTTTGTG; Reverse Primer - name:155217_N_SP6_cDNA_TC1567445, sequence:GTGGTCAGCATCTCACCAAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8959 same embryo
	EMAGE:8958 same embryo
	EMAGE:8962 same embryo
	EMAGE:8960 same embryo
	EMAGE:8963 same embryo
	EurExpress:euxassay_012917 same experiment
	MGI:4829255 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source