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EMAGE:8971

Olfr131 olfactory receptor 131 ( MGI:2177514)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8971 EMAGE:8971 EMAGE:8971 EMAGE:8971 EMAGE:8971
"Pseudo-wholemount" of euxassay_012989. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012989_01 euxassay_012989_02 euxassay_012989_03 euxassay_012989_04
EMAGE:8971 EMAGE:8971 EMAGE:8971 EMAGE:8971 EMAGE:8971
euxassay_012989_05 euxassay_012989_06 euxassay_012989_07 euxassay_012989_08 euxassay_012989_09
EMAGE:8971 EMAGE:8971 EMAGE:8971 EMAGE:8971 EMAGE:8971
euxassay_012989_10 euxassay_012989_11 euxassay_012989_12 euxassay_012989_13 euxassay_012989_14
EMAGE:8971 EMAGE:8971 EMAGE:8971 EMAGE:8971 EMAGE:8971
euxassay_012989_15 euxassay_012989_16 euxassay_012989_17 euxassay_012989_18 euxassay_012989_19
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euxassay_012989_20 euxassay_012989_21 euxassay_012989_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8971Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8971_wholemount_strong.wlz
8971_wholemount_moderate.wlz
8971_wholemount_possible.wlz
8971_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8971_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
spottedstrong expression: see section 14 moderate expression: see section 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39096
Entity Detected:Olfr131, olfactory receptor 131 ( MGI:2177514)
Sequence:sense strand is shown

>T39096
GCCTTGATCCTAAACTCCACACTCCTATGTATTTCTTCCTGGCCAATCTTTCCTTTTTGGATCTCTGCTA
TACCACTTCTACTGTCCCCCAGATGCTAAAAAATATACAGAGCCATGAGAGAAGCATCACCTATGTGGGC
TGCATAGCACAACTGTTCATCTTCCTTAGCTTGGGGTCTACAGAGTGTGTGCTTCTCTCCGTCATGGCCT
TTGATCGCTATGTGGCCATCTGCCAGCCTCTGCGTTACACAGTTATCATGCATCCCCAGCTGTGCCAACA
GCTGGCAGCAGTAGCCTGGATAACTGGTTTCAGCAATTCCTTGGTGCAGACAGTGTTGACTTCTTTGTTA
CCTCGTTGTGGCCAATACCAGATAGAGAATTTCTTCTGTGAGGTACCTGCCATGCTTCAGTTATCATGTG
TTGACACCTGGGTCAATGAAGTAGAGATGTATGCTGCTGTGGTTGTCATAAAAGTTATCCCCCTTGGGCT
AATTCTTTTCTCTTACATAAACATTGTCAGGGCAGTCATAAAGATACAATCTTCTGAAGGTCGAAAGAAG
GCCTTTAACACATGTGGGTCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 152507. Forward Primer - name:152507_F_cDNA_Olfr131, sequence:GCCTTGATCCTAAACTCCACAC; Reverse Primer - name:152507_N_SP6_cDNA_Olfr131, sequence:GAGACCCACATGTGTTAAAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8970 same embryo
 EMAGE:8973 same embryo
 EMAGE:8972 same embryo
 EurExpress:euxassay_012989 same experiment
 MGI:4826877 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS