EMAGE mouse gene expression spatial database

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EMAGE:9148

Tulp4 tubby like protein 4 ( MGI:1916092)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019639. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019639_01	euxassay_019639_02	euxassay_019639_03	euxassay_019639_04

euxassay_019639_05	euxassay_019639_06	euxassay_019639_07	euxassay_019639_08	euxassay_019639_09

euxassay_019639_10	euxassay_019639_11	euxassay_019639_12	euxassay_019639_13	euxassay_019639_14

euxassay_019639_15	euxassay_019639_16	euxassay_019639_17	euxassay_019639_18	euxassay_019639_19

euxassay_019639_20	euxassay_019639_21	euxassay_019639_22	euxassay_019639_23	euxassay_019639_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9148_wholemount_strong.wlz

9148_wholemount_moderate.wlz

9148_wholemount_weak.wlz

9148_wholemount_possible.wlz

9148_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9148_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 01 02 03 15 16 17 18

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22 23 24

midbrain mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

weak

regional

weak expression: see section 04 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 05 06 07 14 17 18 21 weak expression: see section 03 04 15 16 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 15 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 17 18 weak expression: see section 16

trigeminal v nerve

moderate

regional

moderate expression: see section 07 14

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 10 11 12 13 14 17 weak expression: see section 15

neural retina

moderate

regional

moderate expression: see section 01 20 21 22 23

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 04 05 06 07 08 11 13 14 15

vomeronasal organ

weak

regional

weak expression: see section 08 11

lower jaw incisor

weak

regional

weak expression: see section 06 07 11

upper jaw incisor

weak

regional

weak expression: see section 06 07 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55207
Entity Detected:	Tulp4, tubby like protein 4 ( MGI:1916092)
Sequence:	sense strand is shown >T55207 GAAAAAAGTATGTATGCAGCAGTGGAACATGGGCCTGTGCTTTGCAGCGATTCCAACATCCTCTGCCTGT CCTGGAAGGGGCGTGTTCCCAAGAGTGAGAAGGAGAAACCTGTGTGCAGAAGGCGCTACTATGAAGAGGG ATGGTTGGCCACAGGCAATGGGCGAGGTGTGGTGGGAGTGACTTTCACCTCGAGTCACTGTCGCAGAGAT AGGAGTACACCACAGAGAATAAACTTCAACCTGCGAGGCCACAACAGTGAGGTTGTGCTGGTGAGATGGA ATGAGCCATACCAGAAACTGGCCACATGTGATGCAGACGGAGGAATCTTTGTGTGGATTCAATATGAAGG CAGGTGGTCTGTGGAACTGGTCAATGACCGTGGGGCTCAGGTGAGTGATTTCACGTGGAGCCATGATGGG ACTCAAGCTCTCATTTCATATCGAGATGGGTTTGTCCTGGTTGGTTCTGTCAGTGGACAAAGACACTGGT CATCTGAAATCAACCTGGAGAGCCAGATCACCTGTGGTATATGGACTCCCGATGACCAGCAGGTGTTGTT TGGCACAGCAGATGGGCAGGTGATCGTCATGGACTGCCACGGCAGAATGCTAGCCCATGTCCTCCTGCAT GAATCTGATGGCATCCTCAGCATGTCCTGGAATTACCCCATCTTCCTGGTGGAGGACAGCAGTGAGAGTG ACACTGACTCGGATGATTATTCTCCTCCCCAAGATGGGCCCGCGGCTTACCCCATCCCTGTGCAGAACAC CAAGCCGCTGCTCACCGTCAGCTTCACCTCCGGAGACATCAGCTTAATGAACAACTATGACGACTTGTCT CCCACTGTCATCCGTTCAGGGCTGAAAGAGGTGGTGGCCCAGTGGTGTACGCAGGGAGACTTACTGGCAG TTGCTGGGATGGAGCAGCAGGCACAGCTCAGCGAACTGCCCAATGGTCCTCTTCTGAAGAGTGCCATGGT CAAGTTCTACAATGTTAGAGGAGAGCACATTTTCACTCTGGATACACTTGTACAGCGCCCTATCATCTCT ATCTGTTGGGGCCATCGGGACTCTAGGCTGCTTATGGCTTCAGGACCAGCCCTGTATGTGGTTCGTGTGG AACACCGAGTGTCCAGTCTCCAGCTGTTGTGCCAACAGGCCATCGCCAGCACTCTTCGAGAAGACAAGGA TGTTAACAAGCTGACCTTGCCCCCTCGTCTCTGTTCCTACCTCTCCACTGCCTTCATTCCCACTATTAAG CCCCCGATTCCAGACCCCAACAACATGCGAGACTTTGTCAGCTACCCCTCAGCGGGCAATGAACGGCTGC ACTGCACCATGAAGCGCACAGAGGATGACCCAGAGGTCGGCGGGCCCTGCTACACACTCTACTTGGAGTA CCTGGGAGGGCTCGTGCCCATTCTCAAGGGGAGGCGCATCAGCAAACTGCGGCCAGAGTTTGTCATTATG GACCCTCGGACAGACAGCAAATCAGATGAGATCTATGGGAACAGCTTAATTTCTACTGTGATTGACAGCT GCAACTGCTCAGACTCCAGTGATATCGAACTGAGTGATGATTGGGCTGCCAAGAAGTCTCCCAAAATCTC CAGATCTAGCAAATCGCCCAAACTCCCAAGGATCAGCATTGAGGCCCGAAAGTCACCCAAGCTTCCACGG GCTGCTCAAGAGATTTCTCGGTCTCCCCGATTGCCCATGCGCAAGCCCTCCATGGGCTCGCCCAGCCTGA CACGGAGAGAGTTTCCTTTTGAAGACATCACTCAGCACAACTATCTTGCTCAGGTCACATCCAATATCTG GGGAACCAAGTTTAAGATTGTGGGCTTGGCTGCATTCCTACCAACCAACCTCGGTGCAGTAATCTATAAA ACCAGCCTGCTGCATCTCCAGCCTCGGCAGATGACCATTTATCTCCCGGAAGTTAGGAAGATTTCCATGG ACTATATTAACCTGCCTGTCTTCAACCCAAACGTTTTCAGTGAAGATGAGGATGACTTACCAGTTACAGG AGCATCAGGTGTCCCTGAAAACAACCCGCCCTGTACAGTGAACATCCC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4829023 same experiment
	EurExpress:euxassay_019639 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source