EMAGE mouse gene expression spatial database

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EMAGE:9149

Psmg1 proteasome (prosome, macropain) assembly chaperone 1 ( MGI:1860263)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007189. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007189_01	euxassay_007189_02	euxassay_007189_03	euxassay_007189_04

euxassay_007189_05	euxassay_007189_06	euxassay_007189_07	euxassay_007189_08	euxassay_007189_09

euxassay_007189_10	euxassay_007189_11	euxassay_007189_12	euxassay_007189_13	euxassay_007189_14

euxassay_007189_15	euxassay_007189_16	euxassay_007189_17	euxassay_007189_18	euxassay_007189_19

euxassay_007189_20	euxassay_007189_21	euxassay_007189_22	euxassay_007189_23	euxassay_007189_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9149_wholemount_strong.wlz

9149_wholemount_moderate.wlz

9149_wholemount_weak.wlz

9149_wholemount_possible.wlz

9149_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9149_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 20

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 17 weak expression: see section 13 14 15 16

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 14 15 16

olfactory cortex ventricular layer

strong

regional

strong expression: see section 12 13 17 18 19

telencephalon ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 23 moderate expression: see section 04

rest of cerebellum marginal layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 17 18 19 20 21 22 23

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50006
Entity Detected:	Psmg1, proteasome (prosome, macropain) assembly chaperone 1 ( MGI:1860263)
Sequence:	sense strand is shown >T50006 ACTGAGGAGGAAGAAGAGGAGGAGGAGCAGAGCAGGCGGGACACGCCGGAGGACCGGGAGGTCCGGCGGC AGCTGGCGCGGAAGAGGGAGGTTCGGCTTCTCCGAAGACAGACAGAAACATCTCTGGAGGCTGTGCTCCT AGAGACACACCCTTGCTCCAAGTTTATAATTGCAGTAGGAAGCAACGCAACAGCATTCCTGTCAGCGTTT GTTATGAACTCGGGAGTCTGGGAAGAAGTCGGTTGTGCTAAGCTCTGGAATGAATGGTGCAGAACTACAG ACACTGTCCGTCTGTCCCCTACAGATGTTTTCTGTGTGTTTTATCAACTGAAATCAGATCCCTCGGTTTT TCTATGTCAGTGTAGCTGCTACATTGCTGAGGATCAGCAGTTCCAGTGGTTGGAGAAGGTTTTCGGCTTC CAACCCAGAAAGAGCATGCAGGTAACCGTTCTCACATGCCGGCACATCACAGACTACAAGACCCCCGAGT CTACCTGCAGCCTTTCCTCTCCTTTCCTGAGAGCCCTAAAAACTCAGACATTCAAAGATGCCCTCTGCTG CCCACTGCTGGAACAGCCGAACATTGTGCATGACCTGTCTGCAGCAGTTCTGAGCTACTGTCAAGTATGG AAAATCCCTGCGGTTCTGTATCTGTGCTACACTGATGTGATGAAGTTGGACCGTGTCACGGTTGAAGCTT TTAAACCGTTACTTTCTTCCAGGAGCTTGAAATGCTTGGTGAAGAACATTCCTGAAAGCACAGAAATTCT GAAGAAGTTGATGACCACAAATGAGATTCAGAGCAACATTTACACATGA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ACTGAGGAGGAAGAAGAGGAG; Reverse Primer - name:unspecified, sequence:TCATGTGTAAATGTTGCTCTG. The reverse primer contains a 5' extension containing an T7 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using T7 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4827494 same experiment
	EurExpress:euxassay_007189 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source