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EMAGE:916

Lmx1b LIM homeobox transcription factor 1 beta ( MGI:1100513)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:916
Fig 1L Loomis et al, 1998 [PMID:9463360] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: D - dorsal, V - ventral.
Expression Pattern Description
Spatial Annotation:
EMAGE:916EMAGE:916Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
916_voxel_strong_3D_1.wlz
916_voxel_moderate_3D_1.wlz
916_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:916_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hindlimb bud mesenchyme
detected detected
regionalExpression is restricted to the dorsal mesenchyme
forelimb bud mesenchyme
detected detected
regionalExpression is restricted to the dorsal mesenchyme
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1196543
Entity Detected:Lmx1b, LIM homeobox transcription factor 1 beta ( MGI:1100513)
Notes:The probe used in this study by Loomis et al, 1998 [PMID:9463360] is denoted as that used by Cygan et al, 1997 [PMID:9362463] ie. "A plasmid containing the entire coding region of Lmx-1b was linearized with HindIII and transcribed with T7 polymerase to generate a 1.1 kb antisense probe". Exact probe end points are not defined.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
General Information
Authors:Loomis et al, 1998 [PMID:9463360] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9463360] Loomis CA, Kimmel RA, Tong CX, Michaud J, Joyner AL 1998 Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development (125):1137-48
Links:MGI:1339623 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI