EMAGE mouse gene expression spatial database

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EMAGE:9173

Nrip1 nuclear receptor interacting protein 1 ( MGI:1315213)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019638. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019638_01	euxassay_019638_02	euxassay_019638_03	euxassay_019638_04

euxassay_019638_05	euxassay_019638_06	euxassay_019638_07	euxassay_019638_08	euxassay_019638_09

euxassay_019638_10	euxassay_019638_11	euxassay_019638_12	euxassay_019638_13	euxassay_019638_14

euxassay_019638_15	euxassay_019638_16	euxassay_019638_17	euxassay_019638_18	euxassay_019638_19

euxassay_019638_20	euxassay_019638_21	euxassay_019638_22	euxassay_019638_23	euxassay_019638_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9173_wholemount_strong.wlz

9173_wholemount_moderate.wlz

9173_wholemount_weak.wlz

9173_wholemount_possible.wlz

9173_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9173_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 08 09 10 11

sublingual gland primordium

weak

regional

weak expression: see section 06 07 14 15 16

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 weak expression: see section 09 10 12 13

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 06 07 19 20 21 weak expression: see section 01 02 03 04 05 08 09 10 11 15 16 17 18 22 23

olfactory cortex mantle layer

weak

regional

weak expression: see section 09 10 11 14 15 16

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 weak expression: see section 10 11 12 13

spinal cord mantle layer

moderate

regional

moderate expression: see section 07 09 weak expression: see section 06 08 11

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 moderate expression: see section 15 weak expression: see section 09 17 18

tongue muscle

weak

regional

weak expression: see section 11 12 13

stomach

moderate

regional

moderate expression: see section 17 18 19 20 21 22

midgut

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15

lower jaw incisor

moderate

regional

moderate expression: see section 09 10 14 weak expression: see section 13

upper jaw incisor

moderate

regional

moderate expression: see section 09 10 14 15 weak expression: see section 11 16

metanephros

weak

regional

weak expression: see section 03 04 05 06 13 14 15 16

male reproductive system

weak

regional

weak expression: see section 09

urethra of male

weak

regional

weak expression: see section 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55134
Entity Detected:	Nrip1, nuclear receptor interacting protein 1 ( MGI:1315213)
Sequence:	sense strand is shown >T55134 TCTACGGGTCGTTGCTTCATCAGGAAGAGCTGAAGTTTAGCAGGAATGAGCTCGATTATAAATACCCTGC TGGGCATAGTTCAGCCAGCGATGGTGACCACAGGAGTTGGGCCAGAGAGAGCAAAAGCTTCAATGTTCTC AAGCAGCTGCTGCTCTCCGAGAACTGTGTGCGAGATCTGTCCCCACACAGGAGTGACTCTGTCCCCGACA CGAAAAAGAAAGGACACAAAAACAACGCGCCCGGCAGCAAACCTGAATTCGGCATTTCTTCTTTAAATGG ACTGATGTATAGTTCCCCGCAGCCTGGCAGTTGTGTGACGGATCATAGGACATTTTCATACCCGGGAATG GTAAAGACCCCTCTGAGCCCTCCTTTCCCAGAGCACTTGGGCTGTGTGGGGTCCAGACCAGAACCTGGGC TTTTGAATGGATGTTCCGTGCCCGGTGAGAAGGGACCCATTAAGTGGGTCATCACAGATATGGATAAGAA TGAATACGAAAAAGACTCTCCAAGACTGACCAAAACTAATCCGATCCTCTATTACATGCTCCAGAAGGGA GGGGGCAATTCTGTTACCAC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4826792 same experiment
	EurExpress:euxassay_019638 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source