EMAGE mouse gene expression spatial database

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EMAGE:9427

Prrx2 paired related homeobox 2 ( MGI:98218)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019464. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019464_01	euxassay_019464_02	euxassay_019464_03	euxassay_019464_04

euxassay_019464_05	euxassay_019464_06	euxassay_019464_07	euxassay_019464_08	euxassay_019464_09

euxassay_019464_10	euxassay_019464_11	euxassay_019464_12	euxassay_019464_13	euxassay_019464_14

euxassay_019464_15	euxassay_019464_16	euxassay_019464_17	euxassay_019464_18	euxassay_019464_19

euxassay_019464_20	euxassay_019464_21	euxassay_019464_22	euxassay_019464_23	euxassay_019464_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9427_wholemount_strong.wlz

9427_wholemount_moderate.wlz

9427_wholemount_weak.wlz

9427_wholemount_possible.wlz

9427_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9427_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

moderate

spotted

moderate expression: see section 14 15 weak expression: see section 11 12 13 17 18 19

foot skin

moderate

regional

moderate expression: see section 04 05 06 07 08 18 19 20 21 22

vibrissa

strong

regional

strong expression: see section 02 03 moderate expression: see section 04 05 06 18 19 20

nasal septum

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

mandible

moderate

regional

moderate expression: see section 02 03 05 09 10 11 12 weak expression: see section 04 07 08 14 15 16 17 18 19 20

lower jaw incisor

strong

regional

strong expression: see section 10 11 14 15 16 moderate expression: see section 12

lower jaw molar

strong

regional

strong expression: see section 06 07 19

maxilla

moderate

regional

moderate expression: see section 02 05 10 11 weak expression: see section 07 08 14 15 16 17 18

palatal shelf

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

upper jaw incisor

strong

regional

strong expression: see section 09 10 11 14 15 16

upper jaw molar

strong

regional

strong expression: see section 06 07 19

genital tubercle of male

moderate

regional

moderate expression: see section 10 11 12 14 weak expression: see section 13

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 16 17 18 19 20 weak expression: see section 21 22 23

viscerocranium

moderate

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 11 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55163
Entity Detected:	Prrx2, paired related homeobox 2 ( MGI:98218)
Sequence:	sense strand is shown >T55163 CGCAAGAACTTCTCGGTGAGCCACCTCCTGGACCTGGAGGAGGTGGCGGCTGCTGGGCGCAGGGCTGCGG GGCCCGTTTCGGGGCCCGCCGAGGCGCGGGTGGGAGCGGCGCGCGAACCGTCCGGGGGCAGCAGCGGCAC CGAGGCGGCGCCGCAGGACGGTGACTGTCCCAGCCCCGGCCGTGGCACCAAACGAAAGAAGAAGCAGCGC CGGAATCGAACCACATTCAACAGCAGCCAGCTGCAGGCGCTGGAGCGTGTATTTGAGCGCACACACTACC CTGACGCCTTTGTGCGTGAAGAGCTAGCTCGCCGTGTCAACCTCAGTGAGGCACGTGTCCAAGTCTGGTT CCAGAACCGCCGTGCCAAGTTTCGCCGGAATGAACGTGCCATGCTGGCTACCCGCTCTGCCTCGTTGCTC AAGTCTTATGGCCAGGAGGCGGCCATTGAACAGCCTGTGGCCCCCCGACCTACCACGATGAGCCCAGATT ATCTATCCTGGCCAGCATCCTCCCCCTACAGCTCCGTGCCTCCCTATAGCCCCGGAGGTTCAAGTCCTGC AACTCCTGGAGTCAACATGGCCAACAGCATCGCCAGCCTTCGCCTCAAGGCCAAAGAGTTCAGCCTACAC CACAGCCAGGTGCCCACAGTGAACTGA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CGCAAGAACTTCTCGGTGAGC; Reverse Primer - name:unspecified, sequence:TCAGTTCACTGTGGGCACCTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9428 same embryo
	EurExpress:euxassay_019464 same experiment
	MGI:4827453 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source