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EMAGE:954

T brachyury ( MGI:98472)
TS11 (LHF Downs & Davies)
in situ hybridisation

Data Images
EMAGE:954 EMAGE:954
Figure 3G of Lowe et al., 2001 [PMID:11311163] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 3I of Lowe et al., 2001 [PMID:11311163] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image I is a ventral view of the embryo shown in Image G. This is defined as a late head fold (LHF) stage embryo (see Downs & Davies [PMID:8269852] for staging criteria).
Expression Pattern Description
Spatial Annotation:
EMAGE:954Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
954_wholemount_strong_3D_1.wlz
954_wholemount_moderate_3D_1.wlz
954_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:954_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
notochordal plate
detected detected
Expression is detected in the streak, node, notochord and midline
node
detected detected
Expression is detected in the streak, node, notochord and midline
primitive streak
detected detected
Expression is detected in the streak, node, notochord and midline
notochordal process
detected detected
Expression is detected in the streak, node, notochord and midline
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2158745
Entity Detected:T, brachyury ( MGI:98472)
Notes:The T probe used in this study by Lowe et al., 2001 [PMID:11311163] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:129/Sv x C57BL/6J
Age:LHF Downs & Davies
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Lowe et al., 2001 [PMID:11311163] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11311163] Lowe LA, Yamada S, Kuehn MR 2001 Genetic dissection of nodal function in patterning the mouse embryo. Development (128):1831-43
 [ PMID:8269852] Downs KM, Davies T 1993 Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope. Development (118):1255-66
Links:MGI:2159412 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI