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EMAGE:9726

Trim25 tripartite motif-containing 25 ( MGI:102749)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9726 EMAGE:9726 EMAGE:9726 EMAGE:9726 EMAGE:9726
"Pseudo-wholemount" of euxassay_019641. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019641_01 euxassay_019641_02 euxassay_019641_03 euxassay_019641_04
EMAGE:9726 EMAGE:9726 EMAGE:9726 EMAGE:9726 EMAGE:9726
euxassay_019641_05 euxassay_019641_06 euxassay_019641_07 euxassay_019641_08 euxassay_019641_09
EMAGE:9726 EMAGE:9726 EMAGE:9726 EMAGE:9726 EMAGE:9726
euxassay_019641_10 euxassay_019641_11 euxassay_019641_12 euxassay_019641_13 euxassay_019641_14
EMAGE:9726 EMAGE:9726 EMAGE:9726 EMAGE:9726 EMAGE:9726
euxassay_019641_15 euxassay_019641_16 euxassay_019641_17 euxassay_019641_18 euxassay_019641_19
EMAGE:9726 EMAGE:9726 EMAGE:9726 EMAGE:9726
euxassay_019641_20 euxassay_019641_21 euxassay_019641_22 euxassay_019641_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9726Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9726_wholemount_strong.wlz
9726_wholemount_moderate.wlz
9726_wholemount_weak.wlz
9726_wholemount_possible.wlz
9726_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9726_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
adrenal gland
strong strong
regionalstrong expression: see section 05 06 07 08 14 15 16 17
thymus primordium
strong strong
regionalstrong expression: see section 13 moderate expression: see section 08 09 10 11 12
pancreas
moderate moderate
regionalmoderate expression: see section 14 15 16
thyroid gland
strong strong
regionalstrong expression: see section 13 moderate expression: see section 07 weak expression: see section 08 12
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 03 04 05 15 moderate expression: see section 16 17
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 08 09 14 15 17 18 19 weak expression: see section 10 11 12 13 16
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 15 weak expression: see section 12
stomach
moderate moderate
regionalmoderate expression: see section 17 18 19
hindgut
moderate moderate
regionalmoderate expression: see section 11 12
midgut
strong strong
regionalstrong expression: see section 13 14 moderate expression: see section 06 07 08 09 10 11 12 15 16 17 18 19
lower jaw incisor
weak weak
regionalweak expression: see section 10 11 15
lower jaw molar
moderate moderate
regionalmoderate expression: see section 05 18 19
upper jaw incisor
weak weak
regionalweak expression: see section 10 11 15
upper jaw molar
moderate moderate
regionalmoderate expression: see section 05 06 19
liver lobe
strong strong
regionalstrong expression: see section 17 18 19 20 21 22 23 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16
lung
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 13 weak expression: see section 11 12 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55205
Entity Detected:Trim25, tripartite motif-containing 25 ( MGI:102749)
Sequence:sense strand is shown

>T55205
GGAGGAGAAGAGAGTCTACGGAAAATTCGACACCATCTACCAGGTTCTCGTCAAGAAGAAGAGTGAGATG
CAGAAACTGAAGGCAGAGGTTGAGCTCATTATGGACAAAGGGGATGAGTTTGAGTTTCTGGAGAAAGCTG
CAAAATTGCAAGGAGAGTCAACAAAACCAGTCTACATCCCTAAGATAGATCTGGATCATGATTTGATAAT
GGGAATCTACCAAGGGGCGGCGGACCTCAAGAGTGAACTGAAGCACTCCATTAAGAAGCTACAGAAGAAG
TCTGAGGAGCACAATGGCTCAGGTAACAAGGGAGACCAGACACAGTCCACTTTTAAACCCGTACAACCTT
CGAAGAAGACCATTCAAGAAAAGAAGACCAAGAAAACCCCTGTTGCTCCCGGCCCTCCCTCCCACTTCTC
ACCCAACAAGTTGCCCACCTTTGGAGCTCCTGGCCAATCACTGGATTCAAAAGCAACTTCCCCTGATGCC
GCACCCAAAGCCAGTGCTGCACAGCCAGACTCTATAGGAGTCAAGGCTAAGGTGCTAGAAAACTTCTTAA
CCAAGTCCAGAACGGAGCTTCTGGAGTATTTCGTGAAAGTCATCTTCGACTACAATACCGCCCACAACAA
AGTGTCTCTGTCAAACAAGTACACCACCGCCTCTGTGTCTGATGGGCTCCAGCACTACCGGTCCCATCCC
CAGAGGTTCACCTACTGCTCTCAGGTCCTGGGGCTGCACTGCTACAAGAATGGCATTCACTACTGGGAGG
TGGAACTACAGAAGAACAACTTCTGCGGCGTAGGCATCTGCTACGGCAGCATGGAACGGCAGGGCCCCGA
GAGCCGGCTGGGCCGCAACCCCAACTCATGGTGTGTGGAGTGGTTCAATAACAAGATCTCCGCCTGGCAC
AACAACGTGGAGAAGACTCTGCCCTCCACCAAGGCCACTCGAGTCGGCGTGCTGCTCAACTGTGACCACG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GGAGGAGAAGAGAGTCTACG; Reverse Primer - name:unspecified, sequence:CGTGGTCACAGTTGAGCAGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9727 same embryo
 EMAGE:9725 same embryo
 EurExpress:euxassay_019641 same experiment
 MGI:4828911 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS