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EMAGE:9830

Hes5 hairy and enhancer of split 5 (Drosophila) ( MGI:104876)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830
"Pseudo-wholemount" of euxassay_019477. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019477_01 euxassay_019477_02 euxassay_019477_03 euxassay_019477_04
EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830
euxassay_019477_05 euxassay_019477_06 euxassay_019477_07 euxassay_019477_08 euxassay_019477_09
EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830
euxassay_019477_10 euxassay_019477_11 euxassay_019477_12 euxassay_019477_13 euxassay_019477_14
EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830
euxassay_019477_15 euxassay_019477_16 euxassay_019477_17 euxassay_019477_18 euxassay_019477_19
EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830 EMAGE:9830
euxassay_019477_20 euxassay_019477_21 euxassay_019477_22 euxassay_019477_23 euxassay_019477_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9830Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9830_wholemount_strong.wlz
9830_wholemount_moderate.wlz
9830_wholemount_weak.wlz
9830_wholemount_possible.wlz
9830_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9830_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
moderate moderate
regionalmoderate expression: see section 06 07 18 19 20
hypothalamus ventricular layer
strong strong
homogeneousstrong expression: see section 12 13 14 15
diencephalon lateral wall ventricular layer
strong strong
homogeneousstrong expression: see section 12 13 14 15 16
olfactory cortex ventricular layer
strong strong
homogeneousstrong expression: see section 08 09 10 15 16
telencephalon ventricular layer
strong strong
homogeneousstrong expression: see section 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 02
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 07 09 10 11 13 14 15 16 17 18 19 21 22 23 moderate expression: see section 08 12 20 24
pons ventricular layer
strong strong
regionalstrong expression: see section 09 10 11 12 13 16 17 18 19 moderate expression: see section 20 weak expression: see section 14
midbrain ventricular layer
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 20
intermediate grey horn
strong strong
regionalstrong expression: see section 10 moderate expression: see section 11 12 13 14 15 weak expression: see section 16
neural retina
strong strong
regionalstrong expression: see section 01 02 03 04 23 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 10 11 12 weak expression: see section 14 15 16
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 12 weak expression: see section 14
metanephros
moderate moderate
regionalmoderate expression: see section 06 07 08 09 17 18 19 weak expression: see section 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55074
Entity Detected:Hes5, hairy and enhancer of split 5 (Drosophila) ( MGI:104876)
Sequence:sense strand is shown

>T55074
CAAGGAGAAAAACCGACTGCGGAAGCCGGTGGTGGAGAAGATGCGTCGGGACCGCATCAACAGCAGCATA
GAGCAGCTGAAGCTGCTGCTGGAGCAGGAGTTCGCGCGGCACCAGCCCAACTCCAAGCTGGAGAAGGCCG
ACATCCTGGAGATGGCCGTCAGCTACCTGAAACACAGCAAAGCCTTCGCCGCGGCCGCCGGCCCCAAGAG
CCTGCACCAGGACTACAGCGAGGGCTACTCCTGGTGCCTGCAGGAGGCGGTACAGTTCCTGACCCTGCAC
GCCGCCAGCGACACGCAGATGAAGCTGCTTTACCACTTCCAGCGGCCCCCAGCTCCCGCCGCTCCTGCCA
AGGAGCCCCCGGCGCCCGGAGCTGCGCCCCAACCGGCCCGCTCCTCCGCTAAGGCTGCTGCTGCTGCGGT
CTCCACCTCGCGCCAACCCGCCTGCGGCCTCTGGCGGCCCTGGTGACCCAGCGGCCGACCGGTGCCTGGA
GCGGACCAGAGGATGAGCTCGTTCCTCTGGATGTGGGAAGACATTCCCCAGCCGCAGTTCAGCCCCAGGT
TGGCCGCTACCTTCTTCCGAAGGCTCCCTCCTCCGGCTGGCTGCCCAGCAGGAGGGTCATTCTTAGAGAA
TGTGTGTGCAGAGTTGTCATTTGGGGATAATCAGGGCCCACCCTCTGCCGCCCGTCCGACCCCGTGGGGT
TGTTTTGTGTTTGCATTTCAGCAAGTGACTTCTGCGAAGTTCCTGGTCACCACCGGGGGTTCTATGATAT
TTGTAGAGTCGGGGGTTGGCTCACCCCAGCCCGTAGAGGACTTTCTTCAGGGCCCGTTGCTGCTGGGCAA
GCACCCTGCAGGCGGGCTGTGCCCTGGGCACATTTGCCTTTTGTGAAGGCGGAACTGCAGGTGTTTCCTC
ATAGGAAAGCACTCAGCCTGCGTGGGCGTAGGGCCCATGGGGCCTCACCTCAAGGTCCACAT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAAGGAGAAAAACCGACTGC; Reverse Primer - name:unspecified, sequence:ATGTGGACCTTGAGGTGAGG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9831 same embryo
 EurExpress:euxassay_019477 same experiment
 MGI:4825360 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS