EMAGE mouse gene expression spatial database

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EMAGE:9936

Fastkd2 FAST kinase domains 2 ( MGI:1922869)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008173. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008173_01	euxassay_008173_02	euxassay_008173_03	euxassay_008173_04

euxassay_008173_05	euxassay_008173_06	euxassay_008173_07	euxassay_008173_08	euxassay_008173_09

euxassay_008173_10	euxassay_008173_11	euxassay_008173_12	euxassay_008173_13	euxassay_008173_14

euxassay_008173_15	euxassay_008173_16	euxassay_008173_17	euxassay_008173_18	euxassay_008173_19

euxassay_008173_20	euxassay_008173_21	euxassay_008173_22	euxassay_008173_23	euxassay_008173_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9936_wholemount_strong.wlz

9936_wholemount_moderate.wlz

9936_wholemount_weak.wlz

9936_wholemount_possible.wlz

9936_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9936_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 09 10 11 18 19 20 21

thymus primordium

moderate

regional

moderate expression: see section 13 17 weak expression: see section 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20 21

pancreas

weak

regional

weak expression: see section 10 11 12 16

vibrissa

moderate

regional

moderate expression: see section 05 21 22 23 weak expression: see section 06 07

heart ventricle

moderate

spotted

moderate expression: see section 07 08 09 10 11 12 13 16 17 weak expression: see section 14 15

hindgut

moderate

regional

moderate expression: see section 14 15 16 17 18 19

rectum

moderate

regional

moderate expression: see section 16

midgut

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

renal cortex

moderate

regional

moderate expression: see section 09 10 11 12 18 19 21 22 weak expression: see section 20

testis

weak

regional

weak expression: see section 07 08 09 20 21 22 23

urethra of male

weak

regional

weak expression: see section 15 16 17

lung

moderate

regional

moderate expression: see section 07 08 09 10 11 12 24 weak expression: see section 05 06 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T205
Entity Detected:	Fastkd2, FAST kinase domains 2 ( MGI:1922869)
Sequence:	sense strand is shown >T205 CTTAATGCTATAGTGAAGCTGGGAATCCCTCAGAACACTCTTATGGTACAGACTTTGCTGAGGACGATTC AGGAGCGCATCAATGAGTGTGATGAGAGATGTCTTTCAATTTTGTCAACCGCTTTAGTGTCAATGGAGCC ATGCATGAATGTGAATGCGCTTCGAGCAGGGTTGCGAATCCTAGTTGATCAGCAAGTTTGGAACATAAAA CATGTCTTCACCTTACAAACAGTGATGAAATGTATTGGGAAAGACGCACCATCTGCACTTAAGAAGAAAT TGGAGATGAAAGCCTTGAAGGAGTTAGGCAGATTTTCCATCTTGAATAGCCAGCACATGTTTGAAGTCTT AGCAGCTATGGATCTCCGCTCTGTTGTCCTTCTGAATGAATGCAGTAAGGTTGTCATAGATAATGTCCAT GGGTGTCCTTTTAAAGTATTGATCAGCATACTGCAGTCCTGTAGAGACCTCCGATACCAAAATGAAGATC TCTTCAAAAGCATAGCAGAATATG
Notes:	The probe template was PCR amplified from IMAGE:2648623 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2648623 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9932 same embryo
	EMAGE:9934 same embryo
	EMAGE:9933 same embryo
	EMAGE:9935 same embryo
	EurExpress:euxassay_008173 same experiment
	MGI:4824782 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source