EMAGE mouse gene expression spatial database

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EMAGE:9939

Steap3 STEAP family member 3 ( MGI:1915678)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008169. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008169_01	euxassay_008169_02	euxassay_008169_03	euxassay_008169_04

euxassay_008169_05	euxassay_008169_06	euxassay_008169_07	euxassay_008169_08	euxassay_008169_09

euxassay_008169_10	euxassay_008169_11	euxassay_008169_12	euxassay_008169_13	euxassay_008169_14

euxassay_008169_15	euxassay_008169_16	euxassay_008169_17	euxassay_008169_18	euxassay_008169_19

euxassay_008169_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9939_wholemount_strong.wlz

9939_wholemount_moderate.wlz

9939_wholemount_weak.wlz

9939_wholemount_possible.wlz

9939_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9939_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 04 05 06 07 08 moderate expression: see section 09 10 11 weak expression: see section 03

humerus

strong

regional

strong expression: see section 01 02 03 04

femur

strong

regional

strong expression: see section 07 08 09

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 09 10

trigeminal v ganglion

strong

regional

strong expression: see section 08

dorsal root ganglion

strong

single cell

strong expression: see section 09 11 12 13 14 15 16 17 19 20

mandible

strong

regional

strong expression: see section 04 05 06 07 08 09 13 18 19 moderate expression: see section 10 11 12 14 20

maxilla

strong

regional

strong expression: see section 06 07 08 09 13 18 19 moderate expression: see section 10 11 12 14 20

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

liver right lobe

strong

regional

strong expression: see section 14 15 16 17 18 19 20

basisphenoid bone

strong

regional

strong expression: see section 14 15 16 17

vault of skull

strong

regional

strong expression: see section 01 02

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07

clavicle

strong

regional

strong expression: see section 12 13 18 19 20 moderate expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T250
Entity Detected:	Steap3, STEAP family member 3 ( MGI:1915678)
Sequence:	sense strand is shown >T250 GGCTCCTCCAAGCGGCCGGCTGCCGAGGCACTGCTATGTCGGGGGAGATGGACAAGCCGCTGATCAGCCG CCGCCTAGTGGACAGTGATGGCAGTCTGGCTGAGGTCCCCAAGGAGGCCCCCAAAGTGGGCATCCTGGGC AGTGGGGATTTTGCCCGTTCCCTGGCCACACGCCTGGTGGGCTCTGGCTTCAGTGTGGTGGTGGGGAGCC GTAACCCCAAACGCACGGCTGGCCTCTTCCCCTCCTTAGCTCAAGTGACTTTCCAGGAGGAAGCCGTGAG CTCTCCAGAGGTCATCTTTGTGGCCGTGTTCCGGGAGCACTATTCCTCTCTGTGCAGTCTCGCTGACCAG TTGGCTGGCAAGATCCTCGTGGATGTAAGCAACCCCACGGAGAAGGAGCATCTTCAGCACCGCCAGTCTA ACGCTGAGTACCTGGCCTCACTCTTTCCTGCGTGCACTGTGGTGAAGGCCTTCAACGTCATCTCTGCA
Notes:	The probe template was PCR amplified from IMAGE:2651585 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2651585 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9938 same embryo
	EMAGE:9940 same embryo
	EMAGE:9937 same embryo
	EurExpress:euxassay_008169 same experiment
	MGI:4828497 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source