EMAGE mouse gene expression spatial database

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EMAGE:9942

Paqr4 progestin and adipoQ receptor family member IV ( MGI:1923748)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000093. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000093_01	euxassay_000093_02	euxassay_000093_03	euxassay_000093_04

euxassay_000093_05	euxassay_000093_06	euxassay_000093_07	euxassay_000093_08	euxassay_000093_09

euxassay_000093_10	euxassay_000093_11	euxassay_000093_12	euxassay_000093_13	euxassay_000093_14

euxassay_000093_15	euxassay_000093_16	euxassay_000093_17	euxassay_000093_18	euxassay_000093_19

euxassay_000093_20	euxassay_000093_21	euxassay_000093_22	euxassay_000093_23	euxassay_000093_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9942_wholemount_strong.wlz

9942_wholemount_moderate.wlz

9942_wholemount_weak.wlz

9942_wholemount_possible.wlz

9942_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9942_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 03 04 05 06 07

arm

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 01

hindlimb

moderate

regional

moderate expression: see section 06 09

foot

moderate

homogeneous

moderate expression: see section 08 09

leg

moderate

regional

moderate expression: see section 03 04 06 08 09

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 05 06 07 09 10 11 21 22 weak expression: see section 23

vagus x ganglion

moderate

homogeneous

moderate expression: see section 10 11 21 22

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 10 11 22 weak expression: see section 23

spinal component of peripheral nervous system

moderate

homogeneous

moderate expression: see section 11

dorsal root ganglion

moderate

regional

moderate expression: see section 11 19 20 21 22 weak expression: see section 14 23

otic capsule

moderate

regional

moderate expression: see section 04 weak expression: see section 03

inner ear vestibular component

moderate

regional

moderate expression: see section 04 weak expression: see section 03

scapula

moderate

regional

moderate expression: see section 05 06

acetabular region

moderate

regional

moderate expression: see section 05

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T274
Entity Detected:	Paqr4, progestin and adipoQ receptor family member IV ( MGI:1923748)
Sequence:	sense strand is shown >T274 GTTCCTGTCATTCCTGCAGTGAGGATGCAGAGGAGTGGGACCAGGCTTCTCTCAGAGCCAAGTGGACATT GGTCCTGCTTGTATCATCTGGCCAGGAGACAGGAGGGGAACTGCTGCTTTTCCTAGGCAACAGGCACAGC TGTGGAATGGAGGTGTTGGATTCGGGCTTCACTGGACCAAGGACTCAGCTCTTCAGTGCCATGGTCTGAC TGACCTGCCTACCAGAGACTTGTCTGCTCAGGAAATCTCTATACAGTGGGTGGCTCCAGCCTGCTGGCCC AAGGGTACTGACTCGCAGCCAGATCATCCCAAAGGCCCAAGACCCTAGGCAACATCAATAAAGGGACAAG AAGAGCTATGCTGCCACATGAGCAACCTTGGGTGTTCCCAAGACGCATTACTTTTTATTAGACACGGAAG TTTCAGGGGAGAGGTGGGCAAGACGGTCAGAGGTTTAAAAGCACCAAGGCTGGCTGGGCCTGT
Notes:	The probe template was PCR amplified from IMAGE:2655231 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2655231 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9941 same embryo
	EMAGE:9943 same embryo
	EurExpress:euxassay_000093 same experiment
	MGI:4827045 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source