EMAGE mouse gene expression spatial database

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EMAGE:9947

Appl2 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 ( MGI:2384914)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000187. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000187_01	euxassay_000187_02	euxassay_000187_03	euxassay_000187_04

euxassay_000187_05	euxassay_000187_06	euxassay_000187_07	euxassay_000187_08	euxassay_000187_09

euxassay_000187_10	euxassay_000187_11	euxassay_000187_12	euxassay_000187_13	euxassay_000187_14

euxassay_000187_15	euxassay_000187_16	euxassay_000187_17	euxassay_000187_18	euxassay_000187_19

euxassay_000187_20	euxassay_000187_21	euxassay_000187_22	euxassay_000187_23	euxassay_000187_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9947_wholemount_strong.wlz

9947_wholemount_moderate.wlz

9947_wholemount_weak.wlz

9947_wholemount_possible.wlz

9947_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9947_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

strong

regional

strong expression: see section 01 02 04 07 22 23 moderate expression: see section 03

foot

strong

regional

strong expression: see section 05 06 07 22 23 moderate expression: see section 03

adrenal medulla

moderate

homogeneous

moderate expression: see section 09 18

pancreas body parenchyma

strong

spotted

strong expression: see section 10 11 12 16 17

pancreas head parenchyma

strong

spotted

strong expression: see section 09

pancreas tail parenchyma

strong

spotted

strong expression: see section 13 14 15

hypothalamus ventricular layer

strong

regional

strong expression: see section 12 moderate expression: see section 13 weak expression: see section 11

thalamus

moderate

homogeneous

moderate expression: see section 10

thalamus ventricular layer

moderate

regional

moderate expression: see section 11 13 14

cerebral cortex ventricular layer

strong

graded

strong expression: see section 03 04 05 06 07 08 09 10 11 18 19 21 moderate expression: see section 02 15 16 17 20 22 23

corpus striatum

moderate

regional

moderate expression: see section 02 03 04 06 18 weak expression: see section 05 07 08

medulla oblongata basal plate ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 11 13 14 15 moderate expression: see section 12

pons ventricular layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 14 16 17 18 moderate expression: see section 12 13 15

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 14 moderate expression: see section 12 16 weak expression: see section 13

spinal cord ventricular layer

moderate

homogeneous

moderate expression: see section 12 13 weak expression: see section 14

duodenum rostral part epithelium

strong

homogeneous

strong expression: see section 10 11

stomach

strong

homogeneous

strong expression: see section 04 05 moderate expression: see section 07 08 09

foregut-midgut junction epithelium

moderate

homogeneous

moderate expression: see section 13

rectum epithelium

moderate

homogeneous

moderate expression: see section 15

palatal shelf mesenchyme

strong

regional

strong expression: see section 06

upper jaw incisor dental papilla

strong

spotted

strong expression: see section 10 11

upper jaw molar dental papilla

strong

regional

strong expression: see section 06 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T97
Entity Detected:	Appl2, adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 ( MGI:2384914)
Sequence:	sense strand is shown >T97 GAAGCAGAAGACTCTCTTCTGCAGCAGATGTTCATCGTTCGGTTTTTGGGATCAATGGCAGTTAAGACAG ACAGCACGGCTGAAGTGATTTATGAGGCAATGAGACAAGTACTGGCTGCTCGAGCTATTCACAACATCTT CCGCATGACCGAATCCCATCTCATGGTCACCAGTCAAACTCTGAGGTTGATAGATCCTCAGACTCAAGTG TCAAGGGCCTGTTTTGAGCTTACCAGTGTCACACAGTTTGCTGCTCATCAAGAAAACAAAAGACTGGTTG GCTTTGTCATCCGGGTGCCGGAGTCCACAGGAGAAGAGTCTCTGAGCACATACATTTTTGAAAGCAACTC AGAAGGAGAAAAGATATGCTATGCTATTAATTTGGGGAAAGAAATTATTGAGGTTCAAAAGGATCCAGAA GCACTGGCTCGATTAATGCTGTCTGTACCACTAACCAATGATGGAAAGTATGTACTGTTAAACGATCAAG CAGATGACACTGGCGGAAGTCCAAGTGAAAACAGAGGCGCAGAATCTGAAGCATAACT
Notes:	The probe template was PCR amplified from IMAGE:557599 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:557599 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9948 same embryo
	EurExpress:euxassay_000187 same experiment
	MGI:4823198 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source