EMAGE mouse gene expression spatial database

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EMAGE:10229

Pigo phosphatidylinositol glycan anchor biosynthesis, class O ( MGI:1861452)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000094. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000094_01	euxassay_000094_02	euxassay_000094_03	euxassay_000094_04

euxassay_000094_05	euxassay_000094_06	euxassay_000094_07	euxassay_000094_08	euxassay_000094_09

euxassay_000094_10	euxassay_000094_11	euxassay_000094_12	euxassay_000094_13	euxassay_000094_14

euxassay_000094_15	euxassay_000094_16	euxassay_000094_17	euxassay_000094_18	euxassay_000094_19

euxassay_000094_20	euxassay_000094_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10229_wholemount_strong.wlz

10229_wholemount_moderate.wlz

10229_wholemount_weak.wlz

10229_wholemount_possible.wlz

10229_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10229_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 16 17 18 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 04 06 14 15 16 17 18 19 weak expression: see section 05

vagus x ganglion

moderate

regional

moderate expression: see section 04 14 15 16

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 14 15

dorsal root ganglion

strong

regional

strong expression: see section 13 moderate expression: see section 06 07 14 15

metanephros

weak

spotted

weak expression: see section 14

lung

weak

spotted

weak expression: see section 14

nucleus pulposus

strong

regional

strong expression: see section 11

clavicle

moderate

regional

moderate expression: see section 06 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2164
Entity Detected:	Pigo, phosphatidylinositol glycan anchor biosynthesis, class O ( MGI:1861452)
Sequence:	sense strand is shown >T2164 TGGCCTCGANGCCAGATTCGGACGAGGTTCGCCGCTATGGTAATCTCAAGAGTCCTGAGCCCCCTGTGCT CTTTGTGCGCTGGGGGATGCCTCTCATGGTACTGGGCACGGCTGCCTACTGGGCACTGGCATCAGGCGCA GAGGAAGCACCACCACGCCTCCGGGCCCTGGTCGCCGGGGCATCAGCTGTGCTGCCTCGGGCTGTGATGG GGCTGGCTGCCTTGGGGCTCGTGCTGCTGCTCTGGAGGCCTGTGACAGTGCTGGTGAAAGCTGGGGCTGC TACTTCAAGGACCAGGACTATCCTCACTCCCTTCTCAGGTCCCCCTACTTCTCAGGCTGACCTCGACTAT GTAGTCCCACAAATCTACCGCCACATGCAGGAGGAGTTCCAGGGCCGGCTCGAGAGGACTAAAGCTCAGG GTCCCATAACCGTGGCTGCCTATCAGTTGGGAAGCGTGTACTCGGCTGCTATGGTCACGGCCCTCCTCCT CTTGGCCTTCCCACTTCTGCTGTTGCATGTAGAGCGGGTCAGCCTGGTGTTCCT
Notes:	The probe template was PCR amplified from IMAGE:876428 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:876428 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10230 same embryo
	EMAGE:10228 same embryo
	EMAGE:10227 same embryo
	EurExpress:euxassay_000094 same experiment
	MGI:4827202 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source