EMAGE mouse gene expression spatial database

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EMAGE:10431

Tmeff2 transmembrane protein with EGF-like and two follistatin-like domains 2 ( MGI:1861735)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000710. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000710_01	euxassay_000710_02	euxassay_000710_03	euxassay_000710_04

euxassay_000710_05	euxassay_000710_06	euxassay_000710_07	euxassay_000710_08	euxassay_000710_09

euxassay_000710_10	euxassay_000710_11	euxassay_000710_12	euxassay_000710_13	euxassay_000710_14

euxassay_000710_15	euxassay_000710_16	euxassay_000710_17	euxassay_000710_18	euxassay_000710_19

euxassay_000710_20	euxassay_000710_21	euxassay_000710_22	euxassay_000710_23	euxassay_000710_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10431_wholemount_strong.wlz

10431_wholemount_moderate.wlz

10431_wholemount_weak.wlz

10431_wholemount_possible.wlz

10431_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10431_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate

moderate

homogeneous

moderate expression: see section 07 09 10 17 18

facial vii ganglion

strong

homogeneous

strong expression: see section 04 05 06 07 23 moderate expression: see section 21 22

inferior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 07 08 moderate expression: see section 20

superior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 07 08 moderate expression: see section 19 20

trigeminal v ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 07 23 moderate expression: see section 08 19 20 21 22 weak expression: see section 09 18

inferior vagus x ganglion

weak

homogeneous

weak expression: see section 10 18

superior vagus x ganglion

strong

homogeneous

strong expression: see section 09 moderate expression: see section 19

vestibulocochlear viii ganglion cochlear component

strong

homogeneous

strong expression: see section 07

vestibulocochlear viii ganglion vestibular component

strong

homogeneous

strong expression: see section 05 06 07 moderate expression: see section 19 20 21 22

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1719
Entity Detected:	Tmeff2, transmembrane protein with EGF-like and two follistatin-like domains 2 ( MGI:1861735)
Sequence:	sense strand is shown >T1719 TGGCCTCGAGCNAGATTCGGACGAGGCTCACCCAGTCTGGTCGCGCTCTTCTCTTCGGCCGATTGGCCGC CTCCTCTCCGCACCCTGGTTTCTGTCTCCTGCTCCTCCTTTGCCCCGACCCTGTTCCTTATGGCAGCAGC TCCTTGCATCGGGGGCGCAGCTTCTCAGCGGACTATCCTCTCACTCCGCAGGCTGAGCCCGGTCACTGGA TGTTGCTGAAACTCCGGAGATCATGCTCTCCTTTGGTCGCAGTATCACCGCCGGCTGTCCCTGCCGCCGC CCCCTCCTCTGCTGCCGCGGTCCGCGGGATGCTCAGTAGCCCGCTGCGTGGCCCCTGCGATCCCGTGTTC TTCAGAAGCCTTTGGTTGCTGCAGAGTTGCGCGAAGTAGTCATGGTGCTGTGGGAGTCCCCGCGGCAGTG CAGCAGCTGGACACTTTGCGAGGGCTTCTGCTGGCTGCTGCTGCTGCCCGTGACGCTGCTTATCATAGCC CGCCCGGTAAAACTCGCTGCTTTCCCTACCTCCTTAAGTGACTGCCAAACGCCCACCG
Notes:	The probe template was PCR amplified from IMAGE:479316 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:479316 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10432 same embryo
	EurExpress:euxassay_000710 same experiment
	MGI:4828761 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source