EMAGE mouse gene expression spatial database

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EMAGE:10993

Stx18 syntaxin 18 ( MGI:1918366)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001207. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001207_01	euxassay_001207_02	euxassay_001207_03	euxassay_001207_04

euxassay_001207_05	euxassay_001207_06	euxassay_001207_07	euxassay_001207_08	euxassay_001207_09

euxassay_001207_10	euxassay_001207_11	euxassay_001207_12	euxassay_001207_13	euxassay_001207_14

euxassay_001207_15	euxassay_001207_16	euxassay_001207_17	euxassay_001207_18	euxassay_001207_19

euxassay_001207_20	euxassay_001207_21	euxassay_001207_22	euxassay_001207_23	euxassay_001207_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10993_wholemount_strong.wlz

10993_wholemount_moderate.wlz

10993_wholemount_weak.wlz

10993_wholemount_possible.wlz

10993_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10993_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

weak

regional

weak expression: see section 05 06 22 23 24

lower jaw incisor

weak

regional

weak expression: see section 12 13 14 16 17

upper jaw incisor

weak

regional

weak expression: see section 12 13 14 16 17 18

upper jaw molar

weak

regional

weak expression: see section 06 07 08 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4717
Entity Detected:	Stx18, syntaxin 18 ( MGI:1918366)
Sequence:	sense strand is shown >T4717 CCACGCGTCCGCCTCAGCAACCGAGTTCTTGCCGGGGTCGCTAGCTGCTGACGTGTGCCTGGCCGGCTTC AGGCCCTGGGTTGCGGGTCGCTATGGCGGTGGACATCACACTGCTCTTCCGGGCTAGCGTCAAGACAGTG AAGACGCGGAATAAGGCTCTGGGAGTGGCCGTGGGTGGCGGGGCCGATGGCAGCCGGGACGAGCTGTTCC GCAGAAGCCCTCGGCCCAAGGGAGACTTCTCCAGCCGGGCCCGTGAAGTGATTTCTCACATTGGGAAGCT GAGAGATTTTCTCCTGGAGCACAGGAAGGAGTATATTAATGCCTACAGCCACACCATGTCTGACTATGGG AGGATGACAGACACAGAGCGAGACCAGATAGACCAGGATGCTCAGATATTCATAAGGACCTGTTCAGAAG CAATCCAGCAACTCAGAACAGAAGGAGTGTGTAAGCTTTACTCTGAACAGAGAGCCATCCGAGTGAAGCG CGTGGTGGACAAGAAAAGACTATCTAAGCTGGAACCTGAGCCACATACCAAGAGGAAAGACTCCACATCT GAGAAAGCCCCTCAGAACGTTTCCCAGGACTCTGAAGGAAAGCCTGCTGCTGAGGAGCTGCCAGAAAAGC CTTTGGCTGAATCACAGCCCGAACTGGGAACCTGGGGCGATGGCAAGGGTGAAGATGAGCTGTCTCCAGA AGAGATACAGATGTTTGAACAAGAAAATCAGCGACTCATTGGTGAAATGAACAGCTTGTTTGACGAAGTG AGGCAAATCGAAGGGAAAGTCGTTGAAATTTCCAGACTCCAAGAGATATTCACCGAAAAGGTTTTGCAAC AGGAAACTGAGATCGACAGCATTCACCAGTTAGTCGTGGGGGCAACTGAAAATATTAAGGAAGGCAACGA AGACATCCGAGAGGCCATCAAAAACAATGCAGGTTTCCGAGTGTGGATCCTCTTCTTCCTCGTGATGTGT TCCTTTTCCTTGCTCTTCCTCGACTGGTACGACAGCTAGCCCACCAGGGCTGTGTGCCTGCGCCTGCGCC TGCGCCTGTGCCTTGGACGCACCACGTGCCAGCATGTGTCTCATCGGCTGTGGAGCTGTGTAAGGTCCAA GGGCTGGGAAAACACCGCCCCCAAGCCAACCTTTGGAACCCCTCAGGACCTGGAAATACATGGATTGCAA GTGCCCTTCAGAGTGGAAAATAAAAGGCCATCAGGAGCAGCACCTTCAGGACAGGCCACAAGCATGGTGG GGACTGAGCAGAGCAAGGAAATGGCAGGCCTCCTATCTCCATTACAGGCTTGGGGCCCATCTCCTGCCCT TCCCACGCCCGTAGCTTCAGGGGAGAACATGCCTAATGTTGCTAAAGTCATCAGTTCTTATTTCCTTGTG GCATCTAATGTGTGCTGATATATGGCCATTTTGTCATAGACAATAATTCACATCTGCCTTGTCGAGCTAA TAAATGCAATTACAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5035350 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5035350 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10994 same embryo
	EMAGE:10991 same embryo
	EMAGE:10995 same embryo
	EMAGE:10992 same embryo
	EurExpress:euxassay_001207 same experiment
	MGI:4828526 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source