EMAGE mouse gene expression spatial database

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EMAGE:11276

Samd14 sterile alpha motif domain containing 14 ( MGI:2384945)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006624. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006624_01	euxassay_006624_02	euxassay_006624_03	euxassay_006624_04

euxassay_006624_05	euxassay_006624_06	euxassay_006624_07	euxassay_006624_08	euxassay_006624_09

euxassay_006624_10	euxassay_006624_11	euxassay_006624_12	euxassay_006624_13	euxassay_006624_14

euxassay_006624_15	euxassay_006624_16	euxassay_006624_17	euxassay_006624_18	euxassay_006624_19

euxassay_006624_20	euxassay_006624_21	euxassay_006624_22	euxassay_006624_23	euxassay_006624_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11276_wholemount_strong.wlz

11276_wholemount_moderate.wlz

11276_wholemount_weak.wlz

11276_wholemount_possible.wlz

11276_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11276_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 16 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 17 18 19

trigeminal v nerve

strong

regional

strong expression: see section 09 17

spinal cord

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 15 16 17 18

not examined

regional

not examined expression: see section 01 02 03 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 16 17 moderate expression: see section 09 10 11 12 15 18

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 weak expression: see section 12 13

liver right lobe

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7346
Entity Detected:	Samd14, sterile alpha motif domain containing 14 ( MGI:2384945)
Sequence:	sense strand is shown >T7346 CCAGCCCCGCCCGCGCCTCCTGCTTCCCGCAGACCTTCTCCCTGCATCCCTACACACCTCGCCTCTCCAT TCTTTCCGGGACTCTCCCCGCCGATCCCCCACAACACCCTGGAATGGAGCGACTCCAGCCAGCCCGGAGC CCCCCGCGACCGGGGTGAAAGTCCTTCGCCCCTCTCCTGCCCGGCAGTCGCCCCGAACACCATGGGCCTG AGGGGTTCCCGGGGCGCCGGACGCTGAAAATGACGGATGCTGGAGCTCTCTTGAGTCATGGCTTCCTCAA AGCTCCGAGAACCTGTGGATGAAGTTTTTGACCTGGACTTGGCTGTACCAGAGACCACCAGACTGGACAG CAGCTTACACAAAGCCAGGGCTCAACTGCTGGCCAAGGGCCGGAGACACCGGCCTTCCCGTTCCAGGCTA CGAGACAGTGCCAGCTCTGCCGAGGACGGTGAAGGCTCTGATGGGCCCGGAGGCAAGGTGACCGACGGCT GCGGTAGCCCGCTGCACCGGCTGCGCTCGCCTCTACATTCGGGTCCAGGCTCCCCAGCCAGTGGATCTTT CTGCCTGGAGCCCCCGGGCTTGAGGCGCAGTCTGGATGAGGACGAACCGCCGCCCTCGCCACTCGCTCGG TACCGGCCCCTGCACAACGCCGCCTCGCATGAGGGCCTGGCCGCCACCTCCGGCTCGCCACCACGCTCTG CACCCTCCTCCGACAGCTCCCCCAGCTTCGTGCGTCGTTACCCTCGAGCAGAGCCGCACAGCGAAGATGA CAGCCGGGATGCCAGCCCCCCTGAGCCTGCCAGCCCCACCATCGGCTTGGACAAGAAGACTCGCAGGAAA TTCCTGGACCTGGGGGTCACTCTCCGCCGAGCATCCACCAGCAGGAGCAGGAAGGAGAAGGGTAGCAACC GCCTGTCCATGGGCAGCAGGGAGTCTGTAGAAGGTTCTGGAAGGACAGGAAGCTCCCCGTTCCTGCCCTT TTCCTGGTTCACGGACAGCGGCAAGGGCTCTGCTTCCTCGGGGAGTACCACCTCCCCGACCTGTTCCCCC AAACACGAGGGCTTCAGCCCCAAGAAGTCAGCTTCCCAGGAATCTACCCTGAGCGATGACTCCACACCCC CCAGCAGCAGCCCCAAGATCCCCGGTGGTCCTCGACAGGAGACCAAGTGTTCCTATCCCTACCACACGCT GTCCCAGTCTTCGGATGAGTTCCTGGATGAGTCCCTCCCTGCCGTACAACACTGGACCAGCCAGCAGGTG GGCCAGTGGCTACACAGCCTCAACCTGGAGCAGTATGCTGCCGAGTTTGCTGCCCGGCAGGTGGATGGGC CGCAGCTCCTGCAGCTGGACGGAAGCAAACTGAAGAGCCTGGGGCTCAGCAATTCGCATGACCGAGCACT GGTGAAGCGGAAGTTGAAGGAGCTGGCAGCGGCTGCTGAGAAGGAACGGAAGGCGCAGGAGAAGACCGCA AAGCAGCGGGAGAAGCTGCGGCGCCGCGAGAACGATGCCAAGAAGAGCTAGGGATGGCTGGCGGGGCTGG CACCCCGGCGCACTTGCAGCCACCTACATGGGCCTCACCAGGGAGGCAGGGTCCAGGCACCAGGCTCTCG GAGCACGCGGCCCCCCTCTCACCCTGTTTGGACTGAGTCCTCTGGAGGGAGACCCCAGGGGGGAGGGCCC AGTAATAAACCCAATTTCGAGGACTTGTTTGGCGCAGACTTCCAGGGGGAAGCAGGTGGCAGATGAAGAG GGCAGAGGCCAGGAATGCAGCAAACGCGGAGCCCAGAGGAGCCTGGTTCAGGTGGAACCTCCGCCCTCTG ACTAGAGTGGTCCGAGCAGGAAGAAATCTAGCAGGAGAGGCCCTACCATGGCCAGGATTCCTAGGTTTAG AGAGTAGCCTGGGCAACCCGACTGGAGACAGGAGACAGCTGTGTCTCCTCACCTTTTGAAGGTGGCTGTG TGGTGGGAGAACAGATCTCCCCAGAGAGGCCAGTGGGCAGTGCCAGTAGCACTCAGGGTGGATTCCCAGA CACCTCCTCAGAGGAGAGAGAGAGCAGGGCAGGATAAGCTGGCCTCTGTAAGGAAGGCAGAGGGGGAGAA GGGAGCTCTCACTCACTCTCTCTCTCTTCCCCTCCCTCACCTGCAGGTTCCAGCACACAGGTCTGCTCCT CTCCCATTGAGTCCCTTTCGCAGAGCTAAAATCCACTTCTTCGGAGAGTACTTGAGCCTTCGAGGGCAGG AAAAATTGAGAGCTGTTGGGGACACGGGTCACTCCCCCAACTAGTGTCTAGATGGTGAAGAGGCACACCC TAAGTGTGGCCTGCATGCTGCCAGATTGAAAGGGTCCATCAGCTTCCCTGGTGCCCTGGGTGTGTCAAGG CCTCAGAGGCCTGGGGCGGATGGACAGACACTGCTGGCTTAGGTTTGCTAGGCTACAGAGGGGGACTGGG CCAGCCCCGCGTGGGACTGGCACATGTCCTTTGTAGGGCGTGTTCAGAGATAGAGGGTCCAGGTGCAATG CTGACCCAGCTTCTTGGGTGCTGGTCAGTTACTACTAGGCCCAGGTTTTAGATGACAAACCCCCACCCCT CCCAGTTTCCTGGAGAGGTGAACAGGACCTGCATGTTAACTCCCTCTCTCCTGCCTGGGAAAGTGGCTGA AGGCCAGGGCCCTATGTTTGTGCTCAGTCACCTTCACTGGAAGAGGGGGCCTCATAGGGAAAACTAAGGT ATCGGGGCACTGGCACTTCCTAGACTGGGTGGGGAGGGTGGAGGCTCTACTGTCCCCGAAGCCGTGAAGA GCCAAGTGTGCCCACCTGCATGTGAAGGGGGAAGATGGCTATCAACTTCTTTCAATAAATACTTACCACA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191611 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191611 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11275 same embryo
	EMAGE:11277 same embryo
	EurExpress:euxassay_006624 same experiment
	MGI:4827861 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source