EMAGE mouse gene expression spatial database

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EMAGE:11497

Tmcc3 transmembrane and coiled coil domains 3 ( MGI:2442900)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006528. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006528_01	euxassay_006528_02	euxassay_006528_03	euxassay_006528_04

euxassay_006528_05	euxassay_006528_06	euxassay_006528_07	euxassay_006528_08	euxassay_006528_09

euxassay_006528_10	euxassay_006528_11	euxassay_006528_12	euxassay_006528_13	euxassay_006528_14

euxassay_006528_15	euxassay_006528_16	euxassay_006528_17	euxassay_006528_18	euxassay_006528_19

euxassay_006528_20	euxassay_006528_21	euxassay_006528_22	euxassay_006528_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11497_wholemount_strong.wlz

11497_wholemount_moderate.wlz

11497_wholemount_weak.wlz

11497_wholemount_possible.wlz

11497_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11497_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 06 07 08 09 10 21 22 23 moderate expression: see section 04 05 20

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 20

seminiferous cord

strong

regional

strong expression: see section 03 04 05 18 19 20

tail paraxial mesenchyme

strong

regional

strong expression: see section 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6703
Entity Detected:	Tmcc3, transmembrane and coiled coil domains 3 ( MGI:2442900)
Sequence:	sense strand is shown >T6703 GTTGGCCTCCTGGGAGCCAACACAGCTCTGTATAGAAGGCTCCCTGGGACCTGCAGTGTTTAAGAGAGCT CGGCTCACGGCCTTGGGACTACCTGGGTGGCTGACCAGCTCAGGGGTGGGGAGGCCCGAAGAAAGATGAG CAGGCGGTAACTTCGTGATGAGTTTTGCCAGGTGGACCGCCATGACATGAATACCCTCAGCCTACCCCTG AACATACGCCGAGGGGGATCAGACACCAACCTCAACTTCGATGTCCCCGATGGCATCCTGGATTTCCACA AGGTCAAACTCAATGCGGACAGCCTGAGACAAAAAATCTTAAAGGTGACGGAGCAGATAAAGATCGAGCA GACGTCCCGAGATGGGAACGTTGCAGAGTATCTGAAACTGGTCAGCAGTGCAGACAAGCAGCAGGCTGGA CGCATCAAGCAGGTCTTTGAGAAGAAAAACCAGAAGTCAGCCCACTCCATCGCCCAACTTCAGAAGAAGT TGGAGCAGTATCACAGAAAGCTCAGGGAGATCGAACAGAATGGCGTGACCAGGAGCTCAAAGGACATCTC TAAAGACAGCCTGAAGGAAATACACCACTCTCTGAAGGATGCCCACGTGAAATCTCGAACTGCTCCCCAC TGCCTGGAGAGCAGTAAATCTAGCATGCCAGGGGTATCCCTCACGCCCCCCGTGTTTGTCTTCAATAAGT CCAGAGAGTTTGCCAACTTGATCCGGAATAAGTTTGGCAGTGCAGATAACATTGCTCACTTGAAGAATTC CCTGGAAGAGTTCAGGCCCGAAGCCAGCCCCAGGGCTTACGGCGGAAGCGCTACCATCGTGAACAAACCT AAGTACGGCAGCGATGACGAATGTTCTAGTGGCACATCCGGCTCAGCAGACAGCAATGGGAACCAGTCCT TTGGGGCTGGTGGGACCAGCACCCTGGACAGCCAGGGGAAGATTGCCAAGATCATGGAGGAACTGAGGGA GATCAAGGTTACCCAAACCCAGCTGGCGGAAGACATTGAGGCACTGAAGGTGCAGTTTAAGAGAGAATAT GGCTTTATCTCTCAGACTCTGCAAGAGGAGAGATACAGGTATGAGCGACTTGAAGACCAGCTCCATGACC TGACAGAGCTGCACCAGCATGAGACAGCCAACCTGAAGCAGGAGTTGGCCAGCGCCGAGGAGAAGGTGGC CTACCAGGCCTATGAGCGCTCAAGGGACATCCAGGAAGCCTTGGAATCTTGCCAGACCCGCATCTCTAAG CTCGAGCTGCATCAGCAGGAACAGCAGACCCTGCAGACGGATGCCGTGAACGCCAAGGTCCTGCTGGGGA AGTGCATCAACGTGGTTCTGGCCTTCATGACGGTCATCCTGGTGTGTGTGTCCACCCTGGCCAAGTTTGT CTCGCCCATGATGAAGAGCCGCTCCCACATCCTTGGCACCTTCTTTGCTGTGACTCTTCTCGCCATATTC TGTAAAAACTGGGACCATATCTTGTGTGCCATAGAAAGGATAATCATCCCGAGATGAGCCGCTGCTTCCT GCCTTCACATCCTCTTAAAGATTTTATTTTGAAGAAATCCCTGTGCATACTACCAAAATTTCTGAATGGA CGGCTGTGCCAAAGACGGCGCAAGAAGGACCAGAGCTACGTGTGGTGTAAATAAGTAGAGACTCTTAACC CAGTAGCAGATGCCAACACTGTCCCTGTACGTGGTTAACGTGAAACTGTCCCAGAGTCCTCCTCAAGGGG CTCACTGCCTTAATCAGAGCCGATCTCGAGCCCACCTGTTGAGCTCAGCACAGCAAAGCCCTTGTTCTTT GGGCGCCATGATTGCTAACTGCTGTGTTCTGAGTTCCCCCTGACCAGCAAGAATATGGCTAGGACTTGGG GAAGTGAGCAAACAGGAACTGGCAGGGTTGAGCTCCTGGGGGCCTATGGAAGCTCAGTGAGTCTCTCTGT GATCTCTCCTACTTGTCCATGGGTGGCTCTGTGCCCTACTATGTACCCAGTGTTCACAGCGGTCAATGGT GTATGTCCTCATAACGTGGGAATCTTCTACCTGCTTATCCAGCCGTAGTAGTGCACTTTACAAGGTAGAC ACTGGGATTTACAGCCAGGCTTTCCTCTTATGCCCAGAGCACCACACACCTGATTCCATCTTGCGTGGTT AGCAGTGCTCTGCCCTCAGAGCATTCGGCTGGGCTGTTGTCAAACTGTAGCTTGGGTAACTCAGGGTCCT GCGAGCATCCCTGGGGAGGCAGTGCGTTGGCGACTGTTAACTGTGATAAATGCCCTGAGACGACAGGATG CACTTTACGTGATTCACTTTACTTGCGGGTCTGTGGGGATCTGACTGCAAAGCTTGTTCAGGAAATGAAA ACGAATGACGTTCGTGTTACCCAGCTTTCTTTTCTCAGAGACAGCAAAATCAAACTTCATAACTCCAGTG TGTGTGTGTGCTGAGGCAGGACACTGGGGGCCTAAGAGGGATGTGAACTTTTAGGTCTAATCAAATGGTG GCAAGAGTAGTGGGAGCTCTTGACCCAGATGAGAGCAGGAGGGTCCCTTAGGGACGCAAAGGCCATCAGG AATAAAATTAAGTCCTGCGCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3660220 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3660220 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11500 same embryo
	EMAGE:11499 same embryo
	EMAGE:11496 same embryo
	EMAGE:11498 same embryo
	EurExpress:euxassay_006528 same experiment
	MGI:4828755 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source