EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11562

Ren2 renin 2 tandem duplication of Ren1 ( MGI:97899)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006755. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006755_01	euxassay_006755_02	euxassay_006755_03	euxassay_006755_04

euxassay_006755_05	euxassay_006755_06	euxassay_006755_07	euxassay_006755_08	euxassay_006755_09

euxassay_006755_10	euxassay_006755_11	euxassay_006755_12	euxassay_006755_13	euxassay_006755_14

euxassay_006755_15	euxassay_006755_16	euxassay_006755_17	euxassay_006755_18	euxassay_006755_19

euxassay_006755_20	euxassay_006755_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11562_wholemount_strong.wlz

11562_wholemount_moderate.wlz

11562_wholemount_weak.wlz

11562_wholemount_possible.wlz

11562_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11562_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 17 18 19 20 21

adrenal medulla

strong

regional

strong expression: see section 10 15 16

medulla oblongata alar plate mantle layer

strong

single cell

strong expression: see section 04 05 06 07 09 10 11 12 13 14 16 17

metanephros

strong

regional

strong expression: see section 10 11 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7345
Entity Detected:	Ren2, renin 2 tandem duplication of Ren1 ( MGI:97899)
Sequence:	sense strand is shown >T7345 GCCTTGGCTGAACCAGATGGACAGGAGGAGGATGCCTCTCTGGGCACTCTTGTTGCTCTGGAGTCCTTGC ACCTTCAGTCTCCCAACGGGCACCACCTTTGAACGAATCCCACTCAAGAAAATGCCCTCTGTCCGGGAAA TCCTGGAGGAGCGGGGAGTGGACATGACCAGGCTCAGTGCTGAATGGGACGTATTCACAAAGAGGTCTTC CTTGACTGATCTTATCTCCCCCGTGGTCCTCACCAACTACCTGAATAGCCAGTACTATGGCGAGATCGGC ATTGGTACCCCACCCCAGACCTTCAAAGTCATCTTTGACACGGGCTCCGCCAACCTCTGGGTGCCCTCCA CCAAGTGCAGCCGCCTCTACCTTGCTTGTGGGATTCACAGCCTCTATGAGTCCTCTGACTCCTCCAGCTA CATGGAGAATGGAGACGACTTCACCATCCACTACGGATCAGGGAGAGTCAAAGGTTTCCTCAGCCAGGAC TCGGTGACTGTGGGTGGAATCACTGTGACACAGACCTTTGGAGAGGTCACCGAGCTGCCCCTGATCCCTT TCATGCTGGCCCAGTTTGACGGGGTTCTAGGCATGGGCTTTCCCGCTCAGGCCGTCGGCGGGGTCACCCC TGTCTTTGACCACATTCTCTCCCAGGGGGTGCTGAAGGAGAAAGTGTTCTCTGTCTACTACAACAGGGGT CCCCACCTGCTGGGGGGCGAGGTGGTGCTAGGAGGCAGCGACCCGGAGCATTACCAAGGCGATTTTCACT ATGTGAGCCTCAGCAAGACTGATTCCTGGCAGATCACAATGAAGGGGGTGTCTGTGGGGTCTTCCACCCT ACTGTGTGAAGAAGGCTGTGAGGTAGTGGTGGACACTGGTTCATCCTTTATCTCGGCTCCTACGAGCTCC CTGAAGTTGATCATGCAAGCCCTGGGAGCCAAGGAGAAGAGACTACATGAATATGTTGTGAGCTGTAGCC AGGTGCCCACCCTCCCCGACATCTCCTTCAACCTGGGAGGCAGGGCCTACACACTCAGCAGTACGGACTA CGTGCTACAGTATCCCAACAGGAGAGACAAGCTGTGCACAGTGGCTCTCCATGCCATGGACATCCCACCA CCCACTGGGCCTGTCTGGGTCCTGGGTGCCACCTTCATCCGCAAGTTCTATACAGAGTTTGATCGGCATA ACAATCGCATTGGATTCGCCTTGGCCCGCTAAGGCCCTCTGCCACCCAGTAACCCTAGGCCAAGCCAAGC TGGCACTCCTGGGGGCCATTTTGTCTGGCTTTGTCCCCAACATAGGGACACTGGACACAGAGACCCTAAC GAGTGTTTGCCCCTTCACCTGCACTCACCCTTCCCTGCTTTAAGGAAAAACCGAATAAAGATTTCATGTT TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4190969 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4190969 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11564 same embryo
	EMAGE:11563 same embryo
	EMAGE:11560 same embryo
	EMAGE:11561 same embryo
	EurExpress:euxassay_006755 same experiment
	MGI:4827696 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source