EMAGE mouse gene expression spatial database

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EMAGE:11699

AI836003 expressed sequence AI836003 ( MGI:2146066)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006911. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006911_01	euxassay_006911_02	euxassay_006911_03	euxassay_006911_04

euxassay_006911_05	euxassay_006911_06	euxassay_006911_07	euxassay_006911_08	euxassay_006911_09

euxassay_006911_10	euxassay_006911_11	euxassay_006911_12	euxassay_006911_13	euxassay_006911_14

euxassay_006911_15	euxassay_006911_16	euxassay_006911_17	euxassay_006911_18	euxassay_006911_19

euxassay_006911_20	euxassay_006911_21	euxassay_006911_22	euxassay_006911_23	euxassay_006911_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11699_wholemount_strong.wlz

11699_wholemount_moderate.wlz

11699_wholemount_weak.wlz

11699_wholemount_possible.wlz

11699_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11699_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 01 02 03 04 05 06 07 08

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 08 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 17 18

trigeminal v nerve

strong

regional

strong expression: see section 09

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 moderate expression: see section 07 08

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11 16

cervical ganglion

moderate

regional

moderate expression: see section 08 16 17

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 15 16 17

neural retina

moderate

regional

moderate expression: see section 01 02 03 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9187
Entity Detected:	AI836003, expressed sequence AI836003 ( MGI:2146066)
Sequence:	sense strand is shown >T9187 GGGAGTCCCGAGACTGACGCGCTGCGGGTCCCCGTCTCGACCCCGAGCCCTCTGCGAGCGCCTCTGTGAC CTGTGAGCACTCTTTGTCGGTGATTGCCGGGAGGGCGGCGGGGACATAGGTACCACACTGGAGGCGAGCG GGCAAGCCACTTTTGCCCCCTGGGTAAGCCCGAAGATCCTGGTCCCGGGACCCTAGACAGGTCTTCGATC CATAGACTCTTCTCAGCCCTCCTTGATCCCGCCCGTGTCCTCTGCCTGGGGCCGCTCTCTTCACGTAGGC TCCCCGCCCCTCTAGGGAGCCTAGGTGTCCCTGGGCTCCACCCCGTCCCTCCCGGGACCTCTCCCCCTCC ACACCCTTCCCCCACCCTGGAGGCCCGCTGGACACGACCCAGAGCTGCCACCGCCCGCTTCTGCCACTCA ATGGAGGACAGTCTGCTGGAGATCATGACCAAGGACGGCGGCGACATGCCAGCGCCTCTGGAGGTGTCCA CGGTGCCCGCCGTGGGGGACGTGATCTCTGGGGAGTATAACGGCGGTATGAAGGAACTGATGGAGCACCT GAAGGCCCAGCTGCAGGCCCTGTTTGAGGACGTGAGGGCCATGCGAGGGGCACTGGACGAGCAGGCCTCG CACATCCAGGTTCTGTCGGACGACGTGTGCGCCAACCAGCGGGCTATAGTCTCCATGTGCCAGATTATGA CCACTGCGCCCCGCCAGGGAGGCTTGGGCGTGGCCGGCGGCAAGGGGAGCTTCCCAAGTGTTCCCCATGA ACCAGAGACACCTTCGCCTGGGATCGAGGACAGCGGCTTGCTGGGTCGTGATCCTGATGAAGACGAGGAC GAAGAGGAGAAAGAGACACCTAACCCCGCCACACCCACCAGTCACTGCGAGCTCTCTGAGAGCCCCGGTC TCCTTGGGGAGGATGGGCCACTTGTGGAGCCCCCCGACCTTCCTGACATTACCCTGCTGCAGCTGGAGGG CGAGGCCTCTCTGTGAGGGAGACCCCACCAGGGAACCAACTACCCGCGCTGGCTTTGGGTTTCCAAGTGC TTAACCCGAGGGGACTGAACAGTGCGGTGCAGTGTTCTTCACTTCAAACGTGGTCCTTATAGGTCAGGGA GAGAGACTCCCTCACAGAAGGATTTGTAGAGTCAAGGACTTTGGAAGCATCGCGAATTCCACCCAAGCAG GCATCATAGCAAACTGCAGAAAGGTGAGGTTCCCAGAAAGGACGTGCCCATCTCTGGACTCTCATCTATC CCTTTACCTTGCCCCCTTCCCCTCTTCCTAGGAAACAGGAGGACCATTGAATTATGTAAATAAAATTCTG CTTTTTCTATTCTGAAAAAGGAGTCATCTAGGACTTTGGCAAATAATCTCTATTTACCTAGAAATTAAGG AGTTGGGGTATTCTGTTCTGGCAACAGGAAATTTACCCTTTTCCCCAAACCCAAGATATTCAGTGCTCTG CGGAAACCTCTCCCCCTTACAGATCGCTCTGCAACTGCTGATGGGTAAAGGAAGCTTGAGGAGGGAACTG CGGCCCTAGGAGTCAGGGTACTCGGGTTCCCAGGGCCCCTAGGCTGGGAGGAGCTGGCTCTGATGTGCAT GAAGACACAAGAGCTCAAGCTCTGGCACTGAGCATGCAGAGCCGAGCCTGCCCCGTCGCTGACTTCACCT GGGATTGCTTGGCCACTCACCTGGGCCTAGAGAACAAAGTGCTCAGGTCGCAGGCAGAGACTTAGGACTG CTCAGCAACTGTCCAGATTTAAGGGGACATCTGAGGAACTCTTGGGGGAGGGGGCGCTGCAGCCCCAAGC TGTCTTTTCTGGCTATACCTGCTTAACTTCAGTTCCTTTTATTATTTTTTTTTTCCTGTCAATATGTCGG TACCCTCTGCTTTCTGGTGTTGCCTCCATAATGTCTTGTGTTATGTGTGTCCACTTGTGTAGTAGTGTGT GAGCCCTCAGGGGCAGGGCTTGTGGCTCTAGTATTAGGTTCAGGGGGCTTCAGACCCGTCTGCAAGCCCC AGGCTATCATGGCGCTTTCTGCTCTTTTCCCTTTTATGCCCCACTCCTCAACCCAGGGCACGGAGCATGT GGCTGTCCTGAGTGTCCTTAACTGATGTGCTCCTACCTTCCGAAGTGGTGACTGTGTGTGCCCCTTCCCT CCTCTGTGTATCCTTTCTTAGTGCTTTGCTTGATGTCAACCTTAATAAAAAGGCACCATCTCCCCTCTTA AAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6828730 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6828730 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11700 same embryo
	EMAGE:11701 same embryo
	EMAGE:11702 same embryo
	EMAGE:11698 same embryo
	EMAGE:11697 same embryo
	EurExpress:euxassay_006911 same experiment
	MGI:4823047 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source