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EMAGE:11963

Vps13a vacuolar protein sorting 13A (yeast) ( MGI:2444304)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963
"Pseudo-wholemount" of euxassay_008591. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008591_01 euxassay_008591_02 euxassay_008591_03 euxassay_008591_04
EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963
euxassay_008591_05 euxassay_008591_06 euxassay_008591_07 euxassay_008591_08 euxassay_008591_09
EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963
euxassay_008591_10 euxassay_008591_11 euxassay_008591_12 euxassay_008591_13 euxassay_008591_14
EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963
euxassay_008591_15 euxassay_008591_16 euxassay_008591_17 euxassay_008591_18 euxassay_008591_19
EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963 EMAGE:11963
euxassay_008591_20 euxassay_008591_21 euxassay_008591_22 euxassay_008591_23 euxassay_008591_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11963Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11963_wholemount_strong.wlz
11963_wholemount_moderate.wlz
11963_wholemount_weak.wlz
11963_wholemount_possible.wlz
11963_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11963_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36240
Entity Detected:Vps13a, vacuolar protein sorting 13A (yeast) ( MGI:2444304)
Sequence:sense strand is shown

>T36240
GGTTCGTTTAACACCTAGTGGCAATGACATGAAAATTCTGCAACCACATGTGATACCTGTCCGGAGAAAT
TACCTGCCAGCACTAAAAGTAGAATATAACACATCTGCACATCAGTCGTCATTCAGAATTCAGATTTATA
GAATACAGATCCAAAATCAGATTCATGGTGCTATTTTCCCTTTCGTGTTTTATCCTATTAAACCTCCAAG
ATCTGTCACCATGGACTCAGCACCAAAGCCGTTTACAGATGTCAGTATTGTCATGAGATCTGCAGGACAT
TCCCAAATATCACGCATTAAGTATTTCAAAGTGTTGATTCAAGAAATGGACCTCAGTTTGGATCTTGGAT
TTGTGTATGCCCTAGCAGACCTTGTCACAAAAGCTGAAGTGACTGAGAAGACAGAGGTGGAACATTTTCA
TAAGGATGTAGAAGCATTTGAACAAGAATATGAAGTTGTTTCATCAGTAGATCAATCACAAGTCAATCTC
TTTGAATATTTTCATATATCTCCTATCAAGTTGCACTTGAGTGTTTCCCTGAGCTCTGGTAGAGATGAAG
CGAAAGACTCAGAACAACATGGAGGACTAATTCCTGTTCATTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85976. Forward Primer - name:085976_F_cDNA_D330038K10Rik, sequence:GGTTCGTTTAACACCTAGTGGC; Reverse Primer - name:085976_N_SP6_cDNA_D330038K10Rik, sequence:GAATGAACAGGAATTAGTCCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11968 same embryo
 EMAGE:11965 same embryo
 EMAGE:11964 same embryo
 EMAGE:11967 same embryo
 EMAGE:11966 same embryo
 EurExpress:euxassay_008591 same experiment
 MGI:4829167 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS