EMAGE mouse gene expression spatial database

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EMAGE:12551

Lmbrd2 LMBR1 domain containing 2 ( MGI:2444173)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011394. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011394_01	euxassay_011394_02	euxassay_011394_03	euxassay_011394_04

euxassay_011394_05	euxassay_011394_06	euxassay_011394_07	euxassay_011394_08	euxassay_011394_09

euxassay_011394_10	euxassay_011394_11	euxassay_011394_12	euxassay_011394_13	euxassay_011394_14

euxassay_011394_15	euxassay_011394_16	euxassay_011394_17	euxassay_011394_18	euxassay_011394_19

euxassay_011394_20	euxassay_011394_21	euxassay_011394_22	euxassay_011394_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12551_wholemount_strong.wlz

12551_wholemount_moderate.wlz

12551_wholemount_weak.wlz

12551_wholemount_possible.wlz

12551_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12551_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 20

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 20

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 20

tarsus

strong

regional

strong expression: see section 21

femur

strong

regional

strong expression: see section 05 06 20

facial vii ganglion

moderate

regional

moderate expression: see section 04 06 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

meckel's cartilage

strong

regional

strong expression: see section 16 17 18 20 21 moderate expression: see section 02 03 05 19 weak expression: see section 04 06 07 08 09 10 11 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37496
Entity Detected:	Lmbrd2, LMBR1 domain containing 2 ( MGI:2444173)
Sequence:	sense strand is shown >T37496 TTGTTCACACCTTTCAGTCACCTGAGCCAGAAAATCGATTTATCCAATATTTTTATAATCCTACTGTTGA GTGGTACTGGGAATGCCTTTTGCGTCCATGGTTTCACAGGACCCTTGCTGTGGTTTTGTCTATCTTCTCT GTGATTGTTGTGTGGTCAGAGTGTACCTTCTTTAGCACAACTCCTGTCTTATCCCTTTTTGCAGTATTCA TACAGCTGGCAGAAAGAACATACAATTACATTTATATTGAGATTGCTTGCTTCCTTTCCATCTTCTTCTT AAGCATCTGTGTTTACTCTACTGTGTTCAGGATCCGCGTGTTTAACTACTACTACCTGGCCTCACACCAT CAGACCGATGCTTATAGCCTTCTGTTCAGTGGCATGTTATTTTGCCGTTTGACTCCTCCTTTATGTCTTA ACTTCTTGGGATTGACCCATATGGATTCATCCATCTCCCACCAGAATACCCAGCCAACTGCATATACATC TATCATGGGTTCCATGAAAGTTTTATCCTTTATTGCAGATGGATTCTATATATATTATCCTATGTTGGTA GTAATTCTCTGCATCGCTACGTATTTTAGTTTGGGCACCCGTTGTTTGAATCTGCTTGGTTTCCAGCAGT TCATGGGAGATAATGATATGACATCAGATCTAGTTGATGAAGGAAAAGAATTAATCAGACGAGAGAAAAG AAAAAGGCAAAGACAAGAGGAAGGTGAAAATAGAAGAAGAGAGTGGAAAGAACGTTATGGACACAACAGA GAGGATTCCACTCGGAACAGAAACGTCCATCCTGACCCCAAAGAGTCAAATTTCTCAGACACGACTACCA ACTGGTCATCCAAATACACCAGGGCTAATAACAGGACTGAAAGAGACCGAATAGAACTTCTCCAGGATGC TGAGCCTCTGGATTTTAATGCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71677. Forward Primer - name:071677_F_cDNA_9930036E21Rik, sequence:TTGTTCACACCTTTCAGTCACC; Reverse Primer - name:071677_N_SP6_cDNA_9930036E21Rik, sequence:CTGCATTAAAATCCAGAGGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12553 same embryo
	EMAGE:12550 same embryo
	EMAGE:12552 same embryo
	EurExpress:euxassay_011394 same experiment
	MGI:4825943 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source