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EMAGE:12779

Aldh8a1 aldehyde dehydrogenase 8 family, member A1 ( MGI:2653900)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12779 EMAGE:12779 EMAGE:12779 EMAGE:12779 EMAGE:12779
"Pseudo-wholemount" of euxassay_011619. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011619_01 euxassay_011619_02 euxassay_011619_03 euxassay_011619_04
EMAGE:12779 EMAGE:12779 EMAGE:12779 EMAGE:12779 EMAGE:12779
euxassay_011619_05 euxassay_011619_06 euxassay_011619_07 euxassay_011619_08 euxassay_011619_09
EMAGE:12779 EMAGE:12779 EMAGE:12779 EMAGE:12779 EMAGE:12779
euxassay_011619_10 euxassay_011619_11 euxassay_011619_12 euxassay_011619_13 euxassay_011619_14
EMAGE:12779 EMAGE:12779 EMAGE:12779 EMAGE:12779 EMAGE:12779
euxassay_011619_15 euxassay_011619_16 euxassay_011619_17 euxassay_011619_18 euxassay_011619_19
EMAGE:12779 EMAGE:12779 EMAGE:12779 EMAGE:12779
euxassay_011619_20 euxassay_011619_21 euxassay_011619_22 euxassay_011619_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12779Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12779_wholemount_strong.wlz
12779_wholemount_moderate.wlz
12779_wholemount_weak.wlz
12779_wholemount_possible.wlz
12779_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12779_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
liver lobe
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31697
Entity Detected:Aldh8a1, aldehyde dehydrogenase 8 family, member A1 ( MGI:2653900)
Sequence:sense strand is shown

>T31697
ATTGAAGCTGCCGTGGAGGCTGCCAGAGAGGCCTTCCCTGCCTGGTCATCCCGGAGTCCCCAGGAACGTT
CTCTCGTCCTGAACAGGCTGGCTGATGTACTGGAGCAGTCTCTGGAGGAGTTAGCTCAGGCAGAATCTAA
AGACCAAGGGAAAACCCTCACGCTGGCAAGGACCATGGACATTCCTCGGTCTGTTCTGAACTTCCGGTTC
TTCGCTTCCTCCAACCTGCACCACGTATCGGAGTGCACACAGATGAGCCACCTGGGCTGTATGCATTACA
CCGTTCGCACCCCGGTGGGAATTGCTGGTTTGATCAGCCCTTGGAATTTACCACTCTACCTGCTGACCTG
GAAGATAGCTCCGGCCATCGCTGCTGGGAATACCGTGATAGCCAAGCCCAGTGAGATGACTTCTGTGACT
GCCTGGATGTTTTGTAAACTCTTGGATAAAGCAGGTGTCCCACCAGGTGTGATCAACATTGTGTTTGGAA
CAGGGCCGAGGGTTGGTGAAGCCCTTGTGTCTCACCCCGAGGTACCCTTGATCTCGTTCACTGGGAGCCA
GCCCACAGCTGAACGGATTACCCAGCTCAGTGCCCCCCACTGCAAGAAGCTTTCCCTGGAGCTGGGAGGC
AAGAATCCTGCTATCATCTTTGAGGATGCCAACCTGGAGGAGTGTATCCCGGCAACCGTTAGGTCCAGCT
TTGCTAACCAGGGGGAGATCTGCCTTTGTACCAGTAGAATCTTTGTCCAGAGAAGCATCTACAGTGAATT
TCTGAAGAGGTTTGTGGAAGCTACCAGAAAGTGGAAAGTTGGTGTTCCTTCTGACCCATCAGCCAACATG
GGTGCTCTGATAAGCAAAGCACATTTGGAGAAAGTCAGAAGTTATGTCTTGAAAGCTCAGACTGAGGGTG
CCCGGATTCTCTGTGGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4223059), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 33244. Forward Primer - name:033244_F_IRAV81-84_M10_Aldh8a1, sequence:ATTGAAGCTGCCGTGGAG; Reverse Primer - name:033244_R_SP6_IRAV81-84_M10_Aldh8a1, sequence:CACCACAGAGAATCCGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12778 same embryo
 EMAGE:12777 same embryo
 EurExpress:euxassay_011619 same experiment
 MGI:4823086 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS