EMAGE mouse gene expression spatial database

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EMAGE:12963

Lmod1 leiomodin 1 (smooth muscle) ( MGI:2135671)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013697. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013697_01	euxassay_013697_02	euxassay_013697_03	euxassay_013697_04

euxassay_013697_05	euxassay_013697_06	euxassay_013697_07	euxassay_013697_08	euxassay_013697_09

euxassay_013697_10	euxassay_013697_11	euxassay_013697_12	euxassay_013697_13	euxassay_013697_14

euxassay_013697_15	euxassay_013697_16	euxassay_013697_17	euxassay_013697_18	euxassay_013697_19

euxassay_013697_20	euxassay_013697_21	euxassay_013697_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12963_wholemount_strong.wlz

12963_wholemount_moderate.wlz

12963_wholemount_weak.wlz

12963_wholemount_possible.wlz

12963_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12963_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

aorta

weak

regional

weak expression: see section 10 11 12 13

esophagus

weak

regional

weak expression: see section 11 12 13

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

hindgut

moderate

regional

moderate expression: see section 11 12 13

rectum

moderate

regional

moderate expression: see section 12 13

midgut

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

bladder

moderate

regional

moderate expression: see section 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37781
Entity Detected:	Lmod1, leiomodin 1 (smooth muscle) ( MGI:2135671)
Sequence:	sense strand is shown >T37781 TCCTTTAAGCACGTCACAGAGAAGATGGCACTGCCCAGAGCCCATGTAGCAGGCATGCTGCCAAAGGCTT ATGATGCAGAAAAGATGTCTCCCAGGGACCACATTTGGACCCCAGCCTCACTGTCCCCAGGGCCAGGATT CAGGTCTCTGAGGAGCCCTTGGGCAAAGTTGCTGGGCCAGTGGCACTCTATGGGACAGTGGTAGAGGCTG GAGGGGATGAAAGTCCAAAGGCAAAGTAGGAGGGCAGAGGATACTACAAACCCTCCACCTGATATGCCTG GGCAGCAGGCATGGTTGGACAGTGCCTGAGAGAGGGTAGGCTCCAGATGGGAGTAGAAAGACAGACAGAC AGCAGCTGGACCTGAAGGGTTGATGCCAAAGCAGACATTTTCTTCACATCACCTGCTGCTGTATAAATAG CTATATATCTGTTTTTCCATAAGATTTTGATAATATATACAAACCTTTAGCTTCAAGTGGCTATACCTCA CCTTTTTTCATGTTAATGGTCCTAATGTTCCCAATGGTCCCAGGTTCCCTAAAAGGCCTCCCAAAGCTCA GGGTCCCTGGCAAGCCATTCAAGGTGACAGTAGACATCATCCCTAGAGTCCGTGTACCACTACAGAAAGG GCAGTCTGGGGGGAAGCTTGAATTCTTGAGACAACCTTGTGACCTGGTGTTCCAGGTCTGTTCCTCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92034. Forward Primer - name:092034_F_cDNA_Lmod1, sequence:TCCTTTAAGCACGTCACAGAGA; Reverse Primer - name:092034_N_SP6_cDNA_Lmod1, sequence:TGAGGAACAGACCTGGAACAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12964 same embryo
	EMAGE:12962 same embryo
	EMAGE:12965 same embryo
	EurExpress:euxassay_013697 same experiment
	MGI:4825951 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source