EMAGE mouse gene expression spatial database

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EMAGE:13797

Slc2a12 solute carrier family 2 (facilitated glucose transporter), member 12 ( MGI:3052471)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019741. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019741_01	euxassay_019741_02	euxassay_019741_03	euxassay_019741_04

euxassay_019741_05	euxassay_019741_06	euxassay_019741_07	euxassay_019741_08	euxassay_019741_09

euxassay_019741_10	euxassay_019741_11	euxassay_019741_12	euxassay_019741_13	euxassay_019741_14

euxassay_019741_15	euxassay_019741_16	euxassay_019741_17	euxassay_019741_18	euxassay_019741_19

euxassay_019741_20	euxassay_019741_21	euxassay_019741_22	euxassay_019741_23	euxassay_019741_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13797_wholemount_strong.wlz

13797_wholemount_moderate.wlz

13797_wholemount_weak.wlz

13797_wholemount_possible.wlz

13797_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13797_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon

strong

homogeneous

strong expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 04

hindbrain

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 24

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 weak expression: see section 08 09 19 20

trigeminal v nerve

strong

regional

strong expression: see section 10 18

ventral grey horn

strong

regional

strong expression: see section 11 12 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 17 18 weak expression: see section 12

cervical ganglion

moderate

regional

moderate expression: see section 10 18

thoracic ganglion

moderate

regional

moderate expression: see section 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 17 18 19 20

neural retina

strong

regional

strong expression: see section 01 02 03 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56064
Entity Detected:	Slc2a12, solute carrier family 2 (facilitated glucose transporter), member 12 ( MGI:3052471)
Sequence:	sense strand is shown >T56064 GCATTCAAGTCCACCAGTACAAAGATTATGCAGCTTATACATCACACATGAGACACTTGTTAATCAAAGT ACTCTTTTGTAAGTTTTATGTCAATTTTTAAAACTCTAAGATAAACCATTTTCCATTTCTTTTTAAAAAT TGAGTAGAAAGATTAATTTAAAATTCCTGGGACCATATTTATTTAGAAATAGCTGTTAGTAAATATTAGA CAAGGAGTCCGGACATAATATTTATCCAGATCTCTAAGCAGTTAGCTTTAAATTATACAGTAAAAATCTC TAAGAGTTGTCTCCTGGGAAGGCTTTCGTGGTAACCTGTCACAGCCCACCTTCCTGCACTTCTTCCCTGC CCTCAGGAATCACTCTACCTCAGGCTACAGACAGAGGGATGGCCCTGACCTCAGGCCAGGTACCTTCCTT TCTGTGATGAGCAGTGATGGAGAGCCACGCCAGCAAGGTGGCTTTTACCCACGGGATGCGCTTCTCTGGC CTGCTTTTTCCATTTGAGTTTTAAGATTTGACAAAGCATTTCACCGAGAGAATTTGCATGTGATAGAAAA ATAAATCTGTGATTTAAAAAAAAGAGACCAGACCCCCTCCCTAATTTGGGTTTTTCTTCCTTTCACCACC AAATGGTCCCAGTCTGACGAGGTGTGTGCCCGCCACATGCCCTGGTAGCCAAGAGGATGAGGATGGGCAG CAGCAGGAGCCAGCGTAGAGCCTGGGCCTCAGGCGCACAGCAGGAGGCCTGCTTCTCAAGTGCAGCCTCT GAGGAAGGGAGATTTAAACCCTGATCCCATCACAGTGTGTTTCCAACTCGGGAAGAACTGTGCTGGTTCC TTTCTTGAGGGAAGAGGGGGTGAGGTGTGTTTTGTGAAGCTGCACTT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCATTCAAGTCCACCAGTACAA; Reverse Primer - name:unspecified, sequence:CCCTCTTCCCTCAAGAAAGGA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828183 same experiment
	EurExpress:euxassay_019741 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source