EMAGE mouse gene expression spatial database

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EMAGE:13912

Mal2 mal, T-cell differentiation protein 2 ( MGI:2146021)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007855. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007855_01	euxassay_007855_02	euxassay_007855_03	euxassay_007855_04

euxassay_007855_05	euxassay_007855_06	euxassay_007855_07	euxassay_007855_08	euxassay_007855_09

euxassay_007855_10	euxassay_007855_11	euxassay_007855_12	euxassay_007855_13	euxassay_007855_14

euxassay_007855_15	euxassay_007855_16	euxassay_007855_17	euxassay_007855_18	euxassay_007855_19

euxassay_007855_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13912_wholemount_strong.wlz

13912_wholemount_moderate.wlz

13912_wholemount_weak.wlz

13912_wholemount_possible.wlz

13912_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13912_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 12 13 14

thyroid gland

moderate

regional

moderate expression: see section 10 11

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 08 09 12

stomach

moderate

regional

moderate expression: see section 02 03 04 05 weak expression: see section 06 07

rectum

moderate

regional

moderate expression: see section 09

bladder

moderate

regional

Expression in the fundus region.

kidney calyx

strong

regional

strong expression: see section 05 06 07 10 11 12 13 14

urethra of male

weak

regional

weak expression: see section 09 10

left lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11

right lung

strong

regional

strong expression: see section 12 13 14 15 16 17

trachea

moderate

regional

moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2621
Entity Detected:	Mal2, mal, T-cell differentiation protein 2 ( MGI:2146021)
Sequence:	sense strand is shown >T2621 AATTCGGNCGAGGCTCCCCCAGAAGAAGAACCAACGGGGACCTCAGTTTGTAATACAGCTGAAGACGAGA TGTATTTCTATATGTAGGTTGAAGAAATCAGTCTTATGGCTTTCCACTCATGTCTTAGCTCTTCTAAAAT GCCAGACTTCCCTCCTGTTTTTCCAGCTCCAGACCACACACTGAAAAACCTGTATTATGAATTATATACT ATGAAGTCACAAATGTGCCATAAAGATACATTGTACGTGGAATCACTAAAATGTAGTCCACTAGAATTTA GTTGAGATTTTTGTTTTCCCCCAGGTGTACCAAGCCATGCTCAGTGTGGTATTAACTCAAATGAATTCAT TCAGATGTATTGGTGGTACTTTTGTAAATGTATGGCTTCTAGGTTAGCAAAAAGAAAACTCAAAGCTGAC TAACTGTGAATACTTAGGTCTGTAGATTCATTGCATTCTAGATGTTGTGAGCTGAGCGATGAAGCACTTG AACAAAGACGAAGGTGTTTAAGAATCTCTCTACGTATCTTAGCTGTAAAAAT
Notes:	The probe template was PCR amplified from IMAGE:1400700 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1400700 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13916 same embryo
	EMAGE:13915 same embryo
	EMAGE:13911 same embryo
	EMAGE:13914 same embryo
	EMAGE:13913 same embryo
	EurExpress:euxassay_007855 same experiment
	MGI:4826067 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source