EMAGE mouse gene expression spatial database

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EMAGE:13992

Dctpp1 dCTP pyrophosphatase 1 ( MGI:1913672)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007838. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007838_01	euxassay_007838_02	euxassay_007838_03	euxassay_007838_04

euxassay_007838_05	euxassay_007838_06	euxassay_007838_07	euxassay_007838_08	euxassay_007838_09

euxassay_007838_10	euxassay_007838_11	euxassay_007838_12	euxassay_007838_13	euxassay_007838_14

euxassay_007838_15	euxassay_007838_16	euxassay_007838_17	euxassay_007838_18	euxassay_007838_19

euxassay_007838_20	euxassay_007838_21	euxassay_007838_22	euxassay_007838_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13992_wholemount_strong.wlz

13992_wholemount_moderate.wlz

13992_wholemount_weak.wlz

13992_wholemount_possible.wlz

13992_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13992_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

axial musculature

moderate

regional

moderate expression: see section 04 05 06 17 18 19

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

submandibular gland primordium

moderate

regional

moderate expression: see section 08 15 16 17 18 19 weak expression: see section 07

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 weak expression: see section 11

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

mandible

weak

regional

weak expression: see section 03 04 05 06 07 08 10 11 14 15

lower jaw incisor

weak

regional

weak expression: see section 10 11 14 15

lower jaw molar

weak

regional

weak expression: see section 07 08 16 17 18

palatal shelf

weak

regional

weak expression: see section 09

upper jaw incisor

weak

regional

weak expression: see section 10 11 14 15

upper jaw molar

weak

regional

weak expression: see section 07 08 16 18

renal cortex

weak

regional

weak expression: see section 06 07 08 09 14 15 16 17

left lung

weak

spotted

weak expression: see section 03 04 05 06 07 08 09 10 11 12

right lung

moderate

spotted

moderate expression: see section 17 18 19 weak expression: see section 13 14 15 16

orbito-sphenoid

moderate

regional

moderate expression: see section 04 05 06 19 weak expression: see section 01 02 03

midgut loop lumen

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2687
Entity Detected:	Dctpp1, dCTP pyrophosphatase 1 ( MGI:1913672)
Sequence:	sense strand is shown >T2687 GGCCTCNAGCCAGATTCGGCACGAGGGCGGAATGTCCACGGCTGGTGACGGTGAGCGCGGTACTGTGGGA CAAGAAGACTCTGCTGCTGCCCGCCCGTTCAGATTCAGTCCGGAGCCCACGCTTGAGGACATCCGACGCC TCCATGCAGAGTTTGCAGCTGAGCGGGACTGGGAGCAGTTCCACCAGCCTCGGAACCTGCTGCTAGCCTT GGTGGGAGAAGTGGGCGAGCTGGCAGAACTCTTCCAGTGGAAGTCTGATACAGAGCCTGGTCCTCAAGCA TGGCCACCAAAGGAGAGAGCAGCCCTTCAGGAGGAGCTTAGCGACGTCCTCATCTACCTGGTAGCATTAG CAGCCCGCTGCCATGTGGATTTACCTCAAGCAGTAATCTCCAAAATGGACACCAACCGCCAACGTTACCC AGTCCACCTGTCCCGAGGTTCTGCCTGCAAGTACACTGACTTGCCCCGTGGGACCATCTCTGAAACCAGG CTGTGGGGGCTGGAGACCCTGCCTCGGAGCTGAGAGACCAGGCTTCCACATAAAAACGAGGTG
Notes:	The probe template was PCR amplified from IMAGE:1512015 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1512015 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13991 same embryo
	EMAGE:13993 same embryo
	EMAGE:13994 same embryo
	EurExpress:euxassay_007838 same experiment
	MGI:4824257 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source