EMAGE mouse gene expression spatial database

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EMAGE:14232

Ints10 integrator complex subunit 10 ( MGI:1918135)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001760. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001760_01	euxassay_001760_02	euxassay_001760_03	euxassay_001760_04

euxassay_001760_05	euxassay_001760_06	euxassay_001760_07	euxassay_001760_08	euxassay_001760_09

euxassay_001760_10	euxassay_001760_11	euxassay_001760_12	euxassay_001760_13	euxassay_001760_14

euxassay_001760_15	euxassay_001760_16	euxassay_001760_17	euxassay_001760_18	euxassay_001760_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14232_wholemount_strong.wlz

14232_wholemount_moderate.wlz

14232_wholemount_weak.wlz

14232_wholemount_possible.wlz

14232_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14232_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 17 18

telencephalon marginal layer

moderate

regional

moderate expression: see section 15 16 17

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 06 07 08 13 14 15 16

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13

metencephalon rest of alar plate mantle layer

moderate

regional

moderate expression: see section 05 06 07

pons mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

spinal cord mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4172
Entity Detected:	Ints10, integrator complex subunit 10 ( MGI:1918135)
Sequence:	sense strand is shown >T4172 GGGGGTTGGCGGCGCCCTAGTGTCGCCTACTGGAAGGGCAAAAAACCCGGGACCATGTCGGCCCAGGGCG ACTGTGAGTTTCTGGTGCAGCGAGCCCGGGAACTAGTGCCCCAGGACCTGTGGGCAGCCAAGGCATGGCT GATCACGGCCCGCAGCCTCTACCCGGCCGACTTCAACATCCAGTATGAAATGTACACCATCGAACGGAAT GCCGAGAGGACTGCCACCGCAGGGAGGTTGCTGTATGACATGTTTGTGAATTTCCCGGACCAGCCGGTGG TTTGGAGAGAAATCAGCATCATCACGTCAGCGTTGAGGAACGACTCACAAGATAAGCAGACCCAATTCTT ACGGAGTTTATTTGAAACCCTTCCCGGTCGGGTCCAGTGTGAAATGTTGTTGAAGGTGACAGAGCAATGC TTCAACACGCTGGAACGATCAGAAATGTTGCTGCTGCTGCTGAGGCGTTTCCCTGAGACGGTGGTGCAAC ACGGGGTTGGTCTCGGGGAGGCCCTGCTGGAAGCTGAAACCATTGAAGAACAAGACTCTCCTGTTAACTG CTTTAGAAAGCTATTTGTTTGTGATGTCCTTCCTCTAATAATCAACAACCATGATGTCCGCTTGCCCGCC AATCTCTTATATAAATACTTGAACAAAGCAGCTGAATTTTACATCAACTATGTCACTCGGTCCACACAGT CAGAAAATCAGCATCAAGGTGCACAGGATACATCTGATTTAATGTCGCCTAGCAAGCGTAGCTCTCAGAA GTACATAATAGAAGGACTGACTGAGAAATCATCCCACATCGTCGACCCCTGGGAGAGGTTGTTTAAGATT CTGAATGTTGTTGGAATGAGATGCGAATGGCAGATGGATAAAGGGAGACGAAGCTGCAGTGACCTGCTGC ACCGGATGAAGGAGCTCTGCAGATACATGAACAGCTTTGACAGCGAAGCTCACACCAACTATAAAAACCA AGTGCTCTACTCCACGATGCTGGTGTTCTTCAAGAGTGCGTTCCAGTACGTCAGCAGCATACAGCCATCT CTCTTCCAAGGTCCTAACGCCCCAAGCCAGGTTCCACTAGTTCTTCTGGAAGATGTAGCCAATGTGTATG GTGATGTAGAGATTGATCGCAGTAAACACATACACAAGAAGAGGAAGCTAGCTGAGGGAAGAGAAAAAAC CATGAGTTCAGATGACGAAGAGTGTTCAGCGAAAGGCAGAAACCGGCACATTGTTGTCAGCAAAGCTGAC CTCAGCAACTCCATCGAAGTACTGGAGAGCTTCAAACTGGCCAGGGAGAGCTGGGAGTTACTCTACTCTC TGGAATTCCTTGACAAAGAATTTACAAGAATTTGCTTGGCTTGGAAGACAGACACCTGGCTGTGGCTAAG AATCTTCCTCACAGATATGATCATCTACCAGGGTCAATATAAGAAGGCCATAGCCAGTCTGCATCACTTA GCAGCTCTGCAAGGATCCCTTTCTCAGCCACAGATCACAGGACAGGGGACTTTAGAACACCAGAGGGCGC TCATCCAGCTGGCAACGTGTCACTTTGCTCTGGGGGAGTACAGAATGACGTGCGAGAAAGTCCTAGATCT GATGTGTTACATGGTGCTCCCCATACAAGATGGCGGGAAGCCACAGGAAGAGCCGTCCAAAGTAAAGCCC AAGTGTAGAAAAGGTTTGGATCTGAAGCTTCTGCCCTGTACCAGTAAGGCTATCATGCCATACTGCCTGC ACTTGATGCTGGCCTGCTTTAAGCTGAGAGCTTTTACAGACAGCAGAGACGACATGGCGCTGGGCCATGT GATTGTACTGCTCCAGCAGGAGTGGCCACGGGGAGAGAACCTTTTCCTGAAAGCCATCAGTAAGATTTGC CAGCAAGGAAATTTCCAGTATGAGAACTTCTTCAGCTATGTTACAAACATTGATATGCTGGAGGAATTTG CTTATTTAAGAACCCAAGAAGGAGGGAAGATTCATCTGGAATTACTCCCAAATCAAGGAATGCTGATCAA GCACCACACAGTCACTCGAGGCATCACCAAAGGTGTGAAGGAGGACTTCCGCCTGGCCATGGAGCGCCAG GTCTCCCGCTGTGGTGAGAACCTGATGGCGGTACTACATCGCTTCTGCATTAATGAGAAGATCTTGCTCC TGCAGACTCTGACCTGATAGACCTCCTGGCCACCAGCGGCTCAGCCATGACAAGCGCTGCCAGACCAGGG CTGATGAAGAAGAAAAACACCAAGTTCTAACTCCCTTAGTTGCTTAAAAGTCAATCCGAAGAGTATAGGA AGTTATTTCTATTTTTAAGACTTCAGGTTTTTTTAGTTTGTACAAAACAAGAGTCAATCCATTTTGTAAA TAAAAACCCGAAAGATTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4486323 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4486323 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14230 same embryo
	EMAGE:14233 same embryo
	EMAGE:14231 same embryo
	EMAGE:14229 same embryo
	EMAGE:14228 same embryo
	EurExpress:euxassay_001760 same experiment
	MGI:4825606 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source