Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15582

Kcns2 K+ voltage-gated channel, subfamily S, 2 ( MGI:1197011)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15582 EMAGE:15582 EMAGE:15582 EMAGE:15582 EMAGE:15582
"Pseudo-wholemount" of euxassay_010748. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010748_01 euxassay_010748_02 euxassay_010748_03 euxassay_010748_04
EMAGE:15582 EMAGE:15582 EMAGE:15582 EMAGE:15582 EMAGE:15582
euxassay_010748_05 euxassay_010748_06 euxassay_010748_07 euxassay_010748_08 euxassay_010748_09
EMAGE:15582 EMAGE:15582 EMAGE:15582 EMAGE:15582 EMAGE:15582
euxassay_010748_10 euxassay_010748_11 euxassay_010748_12 euxassay_010748_13 euxassay_010748_14
EMAGE:15582 EMAGE:15582 EMAGE:15582 EMAGE:15582 EMAGE:15582
euxassay_010748_15 euxassay_010748_16 euxassay_010748_17 euxassay_010748_18 euxassay_010748_19
EMAGE:15582 EMAGE:15582
euxassay_010748_20 euxassay_010748_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15582Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15582_wholemount_strong.wlz
15582_wholemount_moderate.wlz
15582_wholemount_weak.wlz
15582_wholemount_possible.wlz
15582_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15582_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall mantle layer
weak weak
regionalweak expression: see section 12 14
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 09 10 11 12 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38123
Entity Detected:Kcns2, K+ voltage-gated channel, subfamily S, 2 ( MGI:1197011)
Sequence:sense strand is shown

>T38123
GTCTTGCCCATCACTTTGATCTTCAATAAGTTCTCCCATTTCTATCGGCGCCAAAAGCAACTTGAGAGTG
CTATGCGCAGCTGTGACTTTGGAGATGGAATGAAAGAGGTCCCTTCGGTCAATTTAAGGGACTACTATGC
TCATAAAGTTAAGTCCCTCATGGCAAGTCTGACAAACATGAGTAGGAGTTCACCTAGTGAACTGAGTTTA
GATGATTCTCTACATTAGCTGGACCCCGACTTACATTGCTGATCTGCTGCTTGTGGTTCTAGCACAATCA
GGGCAATTTTAGGGCTGTGGCATAAGAAATCATCCCTGCCCTAGAGGGAGAGCTGCATGGGACATAAGCC
CTAGATTGCTTTTGCAATGTTTAGAGAGGTTTCTTTTTTCTTTTGAGGATGGTGTGTCTAATAACATGCC
TTTGCACCTCTCAGTGAAGTGACACTCACTGGTGTTTGCATCATGGCAAAAAAAAAAATGTTCACCTTTC
TGCCAGATGAGTATCTAGAATGCCAATTTCTCTGTCCACTGTGTACAGTATTCTAATGCTCATATCCCAG
CATTACCTGTGAGTGGAATTGTCTGTGCTCCTATTTCCGAAGCTGCTGTATGGGTTCCAGTGACAACACA
TCTGTCTA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 103592. Forward Primer - name:103592_F_cDNA_Kcns2, sequence:GTCTTGCCCATCACTTTGATCT; Reverse Primer - name:103592_N_SP6_cDNA_Kcns2, sequence:TAGACAGATGTGTTGTCACTGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15580 same embryo
 EMAGE:15584 same embryo
 EMAGE:15579 same embryo
 EMAGE:15581 same embryo
 EMAGE:15583 same embryo
 EurExpress:euxassay_010748 same experiment
 MGI:4825730 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS