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EMAGE:15685

Glra3 glycine receptor, alpha 3 subunit ( MGI:95749)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15685 EMAGE:15685 EMAGE:15685 EMAGE:15685 EMAGE:15685
"Pseudo-wholemount" of euxassay_010858. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010858_01 euxassay_010858_02 euxassay_010858_03 euxassay_010858_04
EMAGE:15685 EMAGE:15685 EMAGE:15685 EMAGE:15685 EMAGE:15685
euxassay_010858_05 euxassay_010858_06 euxassay_010858_07 euxassay_010858_08 euxassay_010858_09
EMAGE:15685 EMAGE:15685 EMAGE:15685 EMAGE:15685 EMAGE:15685
euxassay_010858_10 euxassay_010858_11 euxassay_010858_12 euxassay_010858_13 euxassay_010858_14
EMAGE:15685 EMAGE:15685 EMAGE:15685 EMAGE:15685 EMAGE:15685
euxassay_010858_15 euxassay_010858_16 euxassay_010858_17 euxassay_010858_18 euxassay_010858_19
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euxassay_010858_20 euxassay_010858_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15685Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15685_wholemount_strong.wlz
15685_wholemount_moderate.wlz
15685_wholemount_weak.wlz
15685_wholemount_possible.wlz
15685_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15685_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 10 15 weak expression: see section 11 14 16
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 10 weak expression: see section 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38439
Entity Detected:Glra3, glycine receptor, alpha 3 subunit ( MGI:95749)
Sequence:sense strand is shown

>T38439
TCCTCCAGTTAATGTCACATGCAACATATTCATAAACAGCTTTGGCTCCATTGCAGAGACGACTATGGAT
TACAGAGTAAACATTTTTCTTCGTCAGAAGTGGAATGATCCTCGTCTTGCATACAGTTATCCTGACGATT
CATTAGACCTTGACCCGTCTATGTTGGATTCCATTTGGAAACCTGACTTGTTCTTTGCTAATGAGAAGGG
GGCTAACTTCCACGAAGTCACCACAGATAACAAGCTGCTAAGAATTTTCAAAAATGGAAATGTCCTTTAT
TCAATAAGGTTGACATTAACACTCTCCTGTCCAATGGATCTCAAGAATTTCCCAATGGATGTACAAACAT
GCATAATGCAACTTGAAAGCTTTGGGTACACAATGAATGATCTCATTTTTGAATGGCAAGATGAAGCCCC
AGTACAAGTGGCTGAAGGACTCACTTTGCCTCAATTTCTGTTGAAAGAAGAAAAGGACTTGCGGTACTGC
ACTAAACACTACAATACAGGAAAGTTTACATGCATAGAAGTGCGATTCCATCTTGAGCGTCAGATGGGCT
ATTACTTGATCCAGATGTACATCCCCAGCCTCCTGATCGTTATTCTATCCTGGGTCTCATTCTGGATTAA
CATGGATGCAGCCCCAGCTAGAGTAGCTTTGGGCATCACCACTGATCTTACTATGACCACACAGAGTTCT
GGATCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 148641. Forward Primer - name:148641_F_cDNA_Glra3, sequence:TCCTCCAGTTAATGTCACATGC; Reverse Primer - name:148641_N_SP6_cDNA_Glra3, sequence:GGGATCCAGAACTCTGTGTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15690 same embryo
 EMAGE:15687 same embryo
 EMAGE:15689 same embryo
 EMAGE:15688 same embryo
 EMAGE:15686 same embryo
 EurExpress:euxassay_010858 same experiment
 MGI:4825080 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS