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EMAGE:15763

Akd1 adenylate kinase domain containing 1 ( MGI:2685080)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15763 EMAGE:15763 EMAGE:15763 EMAGE:15763 EMAGE:15763
"Pseudo-wholemount" of euxassay_013265. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013265_01 euxassay_013265_02 euxassay_013265_03 euxassay_013265_04
EMAGE:15763 EMAGE:15763 EMAGE:15763 EMAGE:15763 EMAGE:15763
euxassay_013265_05 euxassay_013265_06 euxassay_013265_07 euxassay_013265_08 euxassay_013265_09
EMAGE:15763 EMAGE:15763 EMAGE:15763 EMAGE:15763 EMAGE:15763
euxassay_013265_10 euxassay_013265_11 euxassay_013265_12 euxassay_013265_13 euxassay_013265_14
EMAGE:15763 EMAGE:15763 EMAGE:15763 EMAGE:15763 EMAGE:15763
euxassay_013265_15 euxassay_013265_16 euxassay_013265_17 euxassay_013265_18 euxassay_013265_19
EMAGE:15763
euxassay_013265_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15763Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15763_wholemount_strong.wlz
15763_wholemount_moderate.wlz
15763_wholemount_weak.wlz
15763_wholemount_possible.wlz
15763_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15763_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 18 19 weak expression: see section 01 02 03 04 05 16 17 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39901
Entity Detected:Akd1, adenylate kinase domain containing 1 ( MGI:2685080)
Sequence:sense strand is shown

>T39901
TCTCTTAGAGGCGAGGTCAATCATTCCCATGGTCATCTTTGAGTTGCATGTGCCGTCCAAGGAGATTTTC
AAAAGACTGCTCTTAGAGAAGAAGACTGAGCAAAGTATGTCTTATCCACTGCACAACAGCTCACAGATCA
TAGCTTATAAGAATGCAAAGTACCATAAAAACATCAATGAGATCAGGCAATTTTATCAAAAGCAGCACCA
AAACTGGCATGTGATTGACGGCTTTCACAGTAAATGGTGGGTATGGAATGAAGTCCTCAAAGACATCCAA
CAGATGAATAAGTACATACAGACATACATGAAAAAAATAAAGGAAGGGAAAGCCGCATGCATTGACAAAT
TATGTATCACGCCCGAGGAGCTGATTGCGCGTCTGGGAGAGTTTAAACAGTTCTGCCCTGTCAGCCTAGC
AGAATCGAATGAACTAATTGATTGCTCCGTAACTAGCAGTTTGGAATTTGCAGCAGAGTTCAGGGGACAC
TACTATAAAATGAGTTCCCAGGAAAAGCTAAATAAATTTCTGAAGGACCCGGAACTGTATGTGCCTCCCT
TGGCACCTCATCCACTCCCACCAGATGACTTGCTTCCCAAAAGGCTTACACTGTCAGAGCTGAAGAGTCG
TTTTCCTAAGTGTGCTGAGCTCCAGGGCTACTGCCCAGTGACTTACCAGGATGGAAAACAAAGATATGAA
GCCCTAGTGCCTGGTAACATTCGATATGCTCTAGAGTATCGTAATTGCATATATATTTGTGAGAGTGAAG
AAAAACTCCAGAAATTCATGAGGTCACCAATGCAATACTGGAACCAGAAGCTTCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 109562. Forward Primer - name:109562_F_cDNA_LOC432456, sequence:TCTCTTAGAGGCGAGGTCAATC; Reverse Primer - name:109562_N_SP6_cDNA_LOC432456, sequence:TGGAAGCTTCTGGTTCCAGTATT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15765 same embryo
 EMAGE:15766 same embryo
 EMAGE:15764 same embryo
 EurExpress:euxassay_013265 same experiment
 MGI:4823063 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS