EMAGE mouse gene expression spatial database

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EMAGE:15880

Mfsd2b major facilitator superfamily domain containing 2B ( MGI:3583946)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013244. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013244_01	euxassay_013244_02	euxassay_013244_03	euxassay_013244_04

euxassay_013244_05	euxassay_013244_06	euxassay_013244_07	euxassay_013244_08	euxassay_013244_09

euxassay_013244_10	euxassay_013244_11	euxassay_013244_12	euxassay_013244_13	euxassay_013244_14

euxassay_013244_15	euxassay_013244_16	euxassay_013244_17	euxassay_013244_18	euxassay_013244_19

euxassay_013244_20	euxassay_013244_21	euxassay_013244_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15880_wholemount_strong.wlz

15880_wholemount_moderate.wlz

15880_wholemount_weak.wlz

15880_wholemount_possible.wlz

15880_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15880_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39919
Entity Detected:	Mfsd2b, major facilitator superfamily domain containing 2B ( MGI:3583946)
Sequence:	sense strand is shown >T39919 CTTCGGAGGAGGACTAGCTACAGCCTTGCCTGAACTTTAAGGAGACCTCTGTGAGCTGGAAAGGAGAAAC CTGCATTTTCTGGAACTCTCTAGCCCACAGCTCCTTGCCTGTTGGACCTGGATCCTTTCAGGCTTGTACC CCAGACTGCTCCACCTTCAAGAAACCTGATTCTAAAAGCATCCTCCTGGAAGGTTTCCAGCATCAGGAAG CCCTGCCTACCCTGGCCCTGTACATTCCTTTTGCTAAATGGCAGAGTTCCAACACAGGAATTCAGACACA GGCCTGGCTTGCATTCACACTCCAGTGGCCACCCGGACACAGTGCAAATGGTCAGGCTGAGGAAACAGGA CATGTTGGAAGCTAAGCAGGTATTTATCCAGCATTTTGCTCTGTTCACACCAGTTGAGGTTACTTCTATC CATAGACAGAGGATGACATGGAACCATCAGAGGCCAGCTACTTTTACAAACAAATTGTGAAATATTCTGA AATTCTATGGTATATATAAAACCATATACTAGGAATTACAGTTTTCTAGGGTCTCTGTCTTTGGGGAAGA AAGATTCACTGGGGTTTATATTTTTTTAAAAAAACAAGTTTAGCTGGGTATGGTGACGCACACATGCAGT CCCAGCATTGGGGAACAGAAGCAGGAAAATCATGAGTTAGAGGCCAGCCTCCACTACATATCAAGTTTGA GGCCAGCCTGAAGAACCTGAGACCCTGTCTTCAAAGCAAATAAAAACTAAAGCTTATTTCCTACCACGTG GGTAAAATGTGACCATGGGTGCCCTACCATCCCATGCTAGAGTCCCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 110042. Forward Primer - name:110042_F_cDNA_LOC432627, sequence:CTTCGGAGGAGGACTAGCTACA; Reverse Primer - name:110042_N_SP6_cDNA_LOC432627, sequence:AGGGACTCTAGCATGGGATGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15876 same embryo
	EMAGE:15879 same embryo
	EMAGE:15881 same embryo
	EMAGE:15877 same embryo
	EMAGE:15878 same embryo
	EurExpress:euxassay_013244 same experiment
	MGI:4826183 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source