EMAGE mouse gene expression spatial database

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EMAGE:15907

Gm15800 predicted gene 15800 ( MGI:3647820)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013370. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013370_01	euxassay_013370_02	euxassay_013370_03	euxassay_013370_04

euxassay_013370_05	euxassay_013370_06	euxassay_013370_07	euxassay_013370_08	euxassay_013370_09

euxassay_013370_10	euxassay_013370_11	euxassay_013370_12	euxassay_013370_13	euxassay_013370_14

euxassay_013370_15	euxassay_013370_16	euxassay_013370_17	euxassay_013370_18	euxassay_013370_19

euxassay_013370_20	euxassay_013370_21	euxassay_013370_22	euxassay_013370_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15907_wholemount_strong.wlz

15907_wholemount_moderate.wlz

15907_wholemount_weak.wlz

15907_wholemount_possible.wlz

15907_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15907_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 17

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 17 18 19 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18

spinal cord

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15

cervical ganglion

strong

regional

strong expression: see section 08 moderate expression: see section 16

thoracic ganglion

moderate

regional

moderate expression: see section 10 12 13

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

neural retina

strong

regional

strong expression: see section 02 03 04 22 23

tongue

strong

spotted

strong expression: see section 12 14

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40021
Entity Detected:	Gm15800, predicted gene 15800 ( MGI:3647820)
Sequence:	sense strand is shown >T40021 GAAAGGAACCGAGACATCATTCGGCGGTCAGGATTGCTTCTTTGGCAGTTGTTGATGGCACCAAAAGATC AAATCTGTCCTGAAATTCAGAAGGAGGTCTGTCTTGCCATCAGCTCTGGTTTAAATATTTTATATCCCGG TGAAACAGAAATTAATAACTTACTTAAGCTGGTCTTAACAGAAGGGGAGAGAAACAGTGGACTCTCCCAG CTCCGTGATGTGATCCTCACCAACCTGGCCGAGCAGCTTCAGAACAACCGATTTGGCAGTGATGAGGACG ATCACTACAGACTCAATGATGAGCTCTTACACTACATCCTGAAGATTGTCGTGCGGGAATCCTGTATCCT AATCACCAAGTGCCAAACGGTCTCCAAGGACGACTTCCAGAAGCTCCTCTCGACGGTGCCTGCTGCATCC TCCTGCCTGCGCTATCTGATGGCGGTTCAGAACCACCTCCTCAGTAACACTGTTTTGATTAAGCCTGATG AGAACGAGGACAGTGACAGCTCCTTGCAGGGAGAGACATTGAAGGAGTTAAAGACCAGTATCTTGGCTCT TGCCACCCAGATCCTGACTGGCTGTGATGAGGTCCTGGAAATGCTGCAGCAGGTCACCACAGCCCTCATA A
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 107857. Forward Primer - name:107857_F_cDNA_LOC433946, sequence:GAAAGGAACCGAGACATCATTC; Reverse Primer - name:107857_N_SP6_cDNA_LOC433946, sequence:TTATGAGGGCTGTGGTGACCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15912 same embryo
	EMAGE:15910 same embryo
	EMAGE:15908 same embryo
	EMAGE:15911 same embryo
	EMAGE:15909 same embryo
	EurExpress:euxassay_013370 same experiment
	MGI:4825089 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source