EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16010

Tmtc1 transmembrane and tetratricopeptide repeat containing 1 ( MGI:3039590)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013458. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013458_01	euxassay_013458_02	euxassay_013458_03	euxassay_013458_04

euxassay_013458_05	euxassay_013458_06	euxassay_013458_07	euxassay_013458_08	euxassay_013458_09

euxassay_013458_10	euxassay_013458_11	euxassay_013458_12	euxassay_013458_13	euxassay_013458_14

euxassay_013458_15	euxassay_013458_16	euxassay_013458_17	euxassay_013458_18	euxassay_013458_19

euxassay_013458_20	euxassay_013458_21	euxassay_013458_22	euxassay_013458_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16010_wholemount_strong.wlz

16010_wholemount_moderate.wlz

16010_wholemount_weak.wlz

16010_wholemount_possible.wlz

16010_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16010_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 13 18 19 weak expression: see section 07 08 09 10 11 15 16 17

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 07

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 03 04 05 06 13 14 18 19 weak expression: see section 07 08 09 10 11 12 15 16 17 20 21

trigeminal v ganglion

weak

single cell

weak expression: see section 04 05 06 07 08 18 19 20

ventral grey horn

weak

regional

weak expression: see section 10 14

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 09 10 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45451
Entity Detected:	Tmtc1, transmembrane and tetratricopeptide repeat containing 1 ( MGI:3039590)
Sequence:	sense strand is shown >T45451 CCAGTCCCAGGATATTAAGCAGTGCTTTGGCAGCAGATAGTGACCTTCATTATTTCTATAGCCCAGAAAT ACAGAAACTCAAAGTCCTGTAAACACAGCAGCTGTTCCTAAGAAAGGATGTGTTCTCTGAAGTCAGCCCT TGAAAATCCGCTTGTGCAACTTCAGTTAATTCTCAACAATCTAGAGTCGGTGGTGAAGAGCATTGGTAGA GCCCTGTGGAGGCAGGGGAGCAGAGCACTTGATAGCTGGGCTTGGGCTAGCATCGCCCAGATTATCACTA AGCACTTTTGGAGAAGTACATTAAATGCTACTCTGGTATATGTTTTTTTCTACTGCCTAAAACACCAAGA TGGACCTCTTGTAGAATTGTATGTGACTATATGTATCTCTACAGGAAAGTCGAAGCTCAGCACCCTTGAT TCTTTATGGAAGTGTTTTAAATGTTTGAACTGTATTCCAGGTTTCACTAGTGTTGTGTCTCCACTCTGAG CCTTCTGAGTGCATTAACACTGAGGTTGGGACCCTGAAATGTGTTGCTCAGGTGTGGAGCTGTGGGCTGC ACTCCTCACAGAAGTGTTAAAGGGTCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 194584. Forward Primer - name:194584_F_exon_BC023818, sequence:CCAGTCCCAGGATATTAAGCAG; Reverse Primer - name:194584_N_SP6_exon_BC023818, sequence:CAGACCCTTTAACACTTCTGTGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16013 same embryo
	EMAGE:16011 same embryo
	EMAGE:16014 same embryo
	EMAGE:16012 same embryo
	EurExpress:euxassay_013458 same experiment
	MGI:4828832 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source