EMAGE mouse gene expression spatial database

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EMAGE:16101

Lama4 laminin, alpha 4 ( MGI:109321)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013553. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013553_01	euxassay_013553_02	euxassay_013553_03	euxassay_013553_04

euxassay_013553_05	euxassay_013553_06	euxassay_013553_07	euxassay_013553_08	euxassay_013553_09

euxassay_013553_10	euxassay_013553_11	euxassay_013553_12	euxassay_013553_13	euxassay_013553_14

euxassay_013553_15	euxassay_013553_16	euxassay_013553_17	euxassay_013553_18	euxassay_013553_19

euxassay_013553_20	euxassay_013553_21	euxassay_013553_22	euxassay_013553_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16101_wholemount_strong.wlz

16101_wholemount_moderate.wlz

16101_wholemount_weak.wlz

16101_wholemount_possible.wlz

16101_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16101_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity

moderate

regional

moderate expression: see section 09 10 11 12 15 16 17 18

lower lip

moderate

regional

moderate expression: see section 11 12 13 14 15 16

upper lip

moderate

regional

moderate expression: see section 11 12 13 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45121
Entity Detected:	Lama4, laminin, alpha 4 ( MGI:109321)
Sequence:	sense strand is shown >T45121 ATCTGTAAGTCCCAGAACCCAAGTCTCAGGAGCGGCGGGAGACCGGAGACAAAGTGAGAGAGGTGGATGG AAGCGAAAGACAAGCAGGAGAGAGAGAAGAGACCACGCTGACTCTCACTCCCTCTTCTTTAATACAACAA AGTTGCAACTTGGGCTCCTGGATCTAAATACCGCAGGTCCAGAGAAGACAAAACACCAGGCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 212817. Forward Primer - name:212817_F_exon_Lama4, sequence:ATCTGTAAGTCCCAGAACCCAA; Reverse Primer - name:212817_N_SP6_exon_Lama4, sequence:TGCCTGGTGTTTTGTCTTCTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16100 same embryo
	EMAGE:16099 same embryo
	EMAGE:16098 same embryo
	EMAGE:16102 same embryo
	EurExpress:euxassay_013553 same experiment
	MGI:4825859 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source