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EMAGE:16104

Otog otogelin ( MGI:1202064)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16104 EMAGE:16104 EMAGE:16104 EMAGE:16104 EMAGE:16104
"Pseudo-wholemount" of euxassay_013561. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013561_01 euxassay_013561_02 euxassay_013561_03 euxassay_013561_04
EMAGE:16104 EMAGE:16104 EMAGE:16104 EMAGE:16104 EMAGE:16104
euxassay_013561_05 euxassay_013561_06 euxassay_013561_07 euxassay_013561_08 euxassay_013561_09
EMAGE:16104 EMAGE:16104 EMAGE:16104 EMAGE:16104 EMAGE:16104
euxassay_013561_10 euxassay_013561_11 euxassay_013561_12 euxassay_013561_13 euxassay_013561_14
EMAGE:16104 EMAGE:16104 EMAGE:16104 EMAGE:16104 EMAGE:16104
euxassay_013561_15 euxassay_013561_16 euxassay_013561_17 euxassay_013561_18 euxassay_013561_19
EMAGE:16104 EMAGE:16104 EMAGE:16104 EMAGE:16104
euxassay_013561_20 euxassay_013561_21 euxassay_013561_22 euxassay_013561_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16104Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16104_wholemount_strong.wlz
16104_wholemount_moderate.wlz
16104_wholemount_weak.wlz
16104_wholemount_possible.wlz
16104_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16104_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cochlea
strong strong
regionalstrong expression: see section 06 07 08 15 16 17 18
saccule epithelium
strong strong
regionalstrong expression: see section 05
utricle
strong strong
regionalstrong expression: see section 02 03 04 06 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45144
Entity Detected:Otog, otogelin ( MGI:1202064)
Sequence:sense strand is shown

>T45144
TAACCTTTACAGGGAGTCCCAACACCACCGTCGCTTCCCGGTCACCCCCTATACCTCGCTTCCCACTCAT
GACCAAAGCTGTGACAGTCCCAAGTCATGACTCCTTTCCTGTTAAAACCACACCTCTGCAGCCCTCTTGG
CTATGGAGCCTCTCTTCCAGACCTATGACTTCCCTTGGAGCTACGTCCTGGCCCCCCACCTCCCCGGGAT
CCCACCTGTCAACTGCAGTAACTAAGGTGGCAAATAAGACAATGACATCCCTGTCTGTCCTGGCCCAGAG
CACTTCAAGTTCCAGCCAGCCCCTGGCTGCAGTGACCACAGCACACAGGGCTCCTGCCAGTCCCCTTGTC
ACCAAGGGCTTGGAGGTGGTGTCAGCCACAGAGAAGGGAGAAGCTGGGCACAGCCAGCTTACAGAGTTGC
CAGTGTCCCCACCCCCAAGCCCAGCCCCCATCGACCTGCCTCACCCAGCCCAACATACCACCACAGCCCC
TGGGCCTTCTGCTCTGTCCCCGGGGATCCTGGCTGCTGGCAGTCCATCCACAGGTGCTCACAGACCGGGG
GCCACAGCTTTAGCTTCTCTAGAGCCAACTCGGCCACCCCACCTTCTCTCTGGCCTACCTCTGGACACCA
GCCTGCCCTTGGCTAAGGTGGGAACCTCTGCCCCAGTGGCCACACCTGGCTCCAAAGGCTATATCCCCAC
CCCTCCACCTCAGCACCAGGCTACAACTTTGGCTACGGCCATGACTGTGAGCCCACTTACTCAGTCACTC
AGTCTGACAGTGCCACTCATGTCTGCTGTAGAGGAACAGGCACACTCACCTAGCCCCAAGCCTCCCCAAG
GAACAGGTATGGCTCCAGACCAGATGCTGGGAGCCACATTGCCAAGCTTTGGAGCCTCCTCTGTGATTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 228487. Forward Primer - name:228487_F_exon_Otog, sequence:TAACCTTTACAGGGAGTCCCAA; Reverse Primer - name:228487_N_SP6_exon_Otog, sequence:CAATCACAGAGGAGGCTCCAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16103 same embryo
 EMAGE:16106 same embryo
 EMAGE:16107 same embryo
 EMAGE:16105 same embryo
 EMAGE:16108 same embryo
 EurExpress:euxassay_013561 same experiment
 MGI:4827003 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS